中国药业2025，Vol.34Issue(8)：50-55,6.DOI:10.3969/j.issn.1006-4931.2025.08.012
紫檀芪对革兰阴性菌致脓毒症模型大鼠肝损伤的改善作用
Improvement Effect of Pterostilbene on Liver Injury in Sepsis Model Rats Induced by Gram-Negative Bacteria
摘要
Abstract
Objective To investigate the improvement effect of pterostilbene on liver injury in sepsis model rats induced by gram-negative bacteria.Methods A total of 40 SD rats were randomly divided into control group(equal volume of normal saline),model group(equal volume of normal saline),PTE group(25 mg/kg)and PTE+EX 527 group(25,5 mg/kg),with 10 rats in each group.Rats in each group were intraperitoneally injected with 109 CFU of Escherichia coli to replicate the sepsis rat model of Gram-negative bacteria.30 minutes before modeling,rats in each group were intraperitoneally injected with corresponding drugs or normal saline.After modeling,the rats were monitored every 6 hours,with continuous observation for 24 hours.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of liver tissue in rats.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and bilirubin(BiL)in serum of rats were detected by electrobiochemical method.The levels of superoxide dismutase(SOD),glutathione(GSH),myeloperoxidase(MPO),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in liver tissue of rats were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA and protein expression levels of SIRT1 and p-STAT3 in rat liver tissues were detected by RT-PCR and Western blot.Results In the model group,the hepatic lobules of rats were destroyed,the liver cells were edematous and degenerated,and there were a large number of inflammatory cell infiltration.In the PTE group,the structure of hepatic lobules tended to be complete,with only a small amount of hepatocyte edema,and the infiltration of inflammatory cells was significantly reduced compared with the model group.The degree of liver tissue injury in the PTE+EX 527 group was similar to that in the model group.Compared with the model group,the levels of AST,ALT,BiL in serum and MPO,IL-6,TNF-α in liver tissue of PTE group were significantly decreased,and the levels of SOD,GSH,the mRNA and protein expression of SIRT1,p-STAT3 and in liver tissue were significantly increased(P<0.05).Compared with PTE group,the levels of AST,ALT,BiL in serum and MPO,IL-6,TNF-α in liver tissue of rats in PTE+EX 527 group were significantly increased,the levels of SOD,GSH,and the mRNA and protein expression of SIRT1,p-STAT3 in liver tissue were significantly decreased(P<0.05).Conclusion Pterostilbene can improve liver injury in septic rats caused by Gram-negative bacteria and inhibit the inflammatory response of hepatocytes.The mechanism may be related to the activation of SIRT1/STAT3 signaling pathway.关键词
紫檀芪/革兰阴性菌/脓毒症/肝损伤/SIRT1/STAT3信号通路Key words
Pterostilbene/Gram-negative bacteria/sepsis/liver injury/SIRT1/STAT3 signaling pathway分类
医药卫生引用本文复制引用
刘建军,曹炜炜,林莫凡,张叶,郭雪梅..紫檀芪对革兰阴性菌致脓毒症模型大鼠肝损伤的改善作用[J].中国药业,2025,34(8):50-55,6.基金项目
上海市虹口区卫生健康委员会中医药课题立项建设项目[HKQ-ZYY-2020-46]. （）
