浙江大学学报（农业与生命科学版）2025，Vol.51Issue(2)：252-265,14.DOI:10.3785/j.issn.1008-9209.2024.07.121
基于比较转录组发掘控制大麦根毛长度的基因
Comparative transcriptome reveals genes regulating root hair length of barley
摘要
Abstract
Root hairs,the primary sites for water and nutrient uptake,make barley—with its long root hair traits and strong environmental adaptability—an ideal candidate for investigating root hair regulatory genes.In this study,by analyzing the transcriptome of barley roots which exhibiting significant differences in root hair length,we identified 274 shared differentially expressed genes.Further analyses via Gene Ontology(GO)functional annotation,Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment,MapMan metabolic pathway analysis,and protein-protein interaction(PPI)network prediction led to the identification of a set of genes regulating root hair length,with higher expression levels in long root hair genotypes than in short ones.These genes primarily encompass genes involved in hormone signaling pathways,including the auxin,ethylene,and salicylic acid pathways,as well as secondary metabolism pathways,such as the phenylpropanoid,lignin,and lignan pathways.They also include genes related to peroxidase and glutathione S-transferase that maintaining reactive oxygen species(ROS)homeostasis,ubiquitin pathway,receptor kinases,and calcium signal transduction.The results from this study shed initial light on the molecular mechanisms controlling barley root hair length and also provide candidate genes for the genetic improvement of crop root traits.关键词
大麦/根毛长度/转录组测序/差异表达基因/代谢通路Key words
barley/root hair length/transcriptome sequencing/differentially expressed genes/metabolic pathways分类
农业科技引用本文复制引用
贺小彦,王永梅,何东,吴港,孙庆译,孙雪莲,刘文星,曾建斌..基于比较转录组发掘控制大麦根毛长度的基因[J].浙江大学学报（农业与生命科学版）,2025,51(2):252-265,14.基金项目
国家自然科学基金面上项目(32171976) （32171976）
山东省自然科学基金面上项目(ZR2021MC077) （ZR2021MC077）
中央引导地方科技发展资金项目(YDZX2021078). （YDZX2021078）
