浙江大学学报（农业与生命科学版）2025，Vol.51Issue(2)：266-276,11.DOI:10.3785/j.issn.1008-9209.2024.12.311
短童期百合营养生长阶段转变的重要通路筛选
Screening of key candidate regulatory pathways during the vegetative phase change in lily with short juvenile stage
摘要
Abstract
Lily(Lilium spp.)is one of the most representative bulbous flowers.However,the vast majority of lilies have a long juvenile stage,which is a significant bottleneck for the development of the entire production industry.In this study,we analyzed the transition from the juvenile stage to the adult stage of different germplasms and bulblet sizes in lily.Furthermore,using the typical short-juvenile-stage germplasm L.×formolongi seedlings as materials,second-generation transcriptome sequencing was performed with shoot apical meristem(SAM)samples from the juvenile stage(0-4 months),transition stage(4-6 months)and adult stage(6-24 months)plants.The transcriptome data were assembled and annotated,and 15 172 differentially expressed genes were identified.Additionally,key candidate regulatory pathways involved in the transition from the juvenile stage to the adult stage of L.×formolongi,e.g.,carbohydrate metabolism and regulation of post-embryonic development,were identified.Real-time fluorogenic quantitative polymerase chain reaction(qRT-PCR)was used to verify the expression patterns of six differentially expressed genes during the transition from the juvenile stage to the adult stage.The results showed that the expression trends of the selected genes at different developmental stages were basically consistent with the transcriptome sequencing results,with a coefficient of determination of 0.913 0,demonstrating the reliability of the gene expression data obtained from transcriptome sequencing.In conclusion,based on the use of specific high-quality resources,the generated transcriptome data reflecting the growth process of seedlings lay a foundation for the construction of co-expression networks and core gene mining in the future.关键词
新铁炮百合/短童期/营养生长阶段转变/通路筛选/差异种质/胚后发育调控Key words
Lilium×formolongi/short juvenile stage/vegetative phase change/pathway screening/distinctive germplasm/regulation of post-embryonic development分类
农业科技引用本文复制引用
郭振峰,徐蕴晨,梅宏建,康婕,杨中振,杨燕,夏宜平,吴昀..短童期百合营养生长阶段转变的重要通路筛选[J].浙江大学学报（农业与生命科学版）,2025,51(2):266-276,11.基金项目
国家自然科学基金青年科学基金项目(32002071) （32002071）
国家自然科学基金面上项目(32372743) （32372743）
浙江农林大学科研启动基金项目(2024LFR125) （2024LFR125）
杭州市农业与社会发展科研引导项目(20220919Y1). （20220919Y1）
