浙江大学学报（农业与生命科学版）2025，Vol.51Issue(2)：277-290,14.DOI:10.3785/j.issn.1008-9209.2025.02.111
二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响
Effect of quinclorac-resistant Echinochloa spp.on the microbiota structure and function of Laodelphax striatellus
摘要
Abstract
Quinclorac-resistant Echinochloa spp.can promote the growth and reproduction of Laodelphax striatellus,which is closely related to the microbiota in vivo.In this study,16S rRNA gene amplicon sequencing was used to analyze and compare the structural characteristics of microbiota in Laodelphax striatellus fed on quinclorac-resistant and sensitive Echinochloa spp.and rice,and to predict the functions of the bacterial communities,in order to investigate the effect of quinclorac-resistant Echinochloa spp.on the structure and function of microbiota in Laodelphax striatellus.The results showed that feeding on quinclorac-resistant Echinochloa spp.reduced the relative abundance of Proteobacteria microbiota but increased that of Bacteroidota microbiota.At the genus level,the relative abundance of Wolbachia microbiota exhibited a declining trend,whereas Chryseobacterium and Stenotrophomonas microbiota showed increasing trends in relative abundance,although no significant differences in alpha diversity index were observed.Functional prediction analysis revealed that the in vivo bacterial communities in Laodelphax striatellus fed on quinclorac-resistant and sensitive Echinochloa spp.exhibited significantly elevated gene abundances in pathways related to energy metabolism,immune system,and nervous system,whereas resistance-related gene abundance decreased in those fed on quinclorac-resistant Echinochloa crus-pavonis.The results of this study demonstrate that feeding on quinclorac-resistant Echinochloa spp.alters the microbiota structure and affects the gene function of microbiota in Laodelphax striatellus,providing a theoretical foundation for elucidating the dynamic regulatory mechanism underlying the response of the microbiota in Laodelphax striatellus to quinclorac-resistant Echinochloa spp.关键词
除草剂抗性/稗草/灰飞虱/微生物群落Key words
herbicide resistance/Echinochloa spp./Laodelphax striatellus/microbiota分类
生物科学引用本文复制引用
郭嘉雯,皮艺萌,鲁艳辉,杨霞,许益鹏,吕仲贤,李永丰,徐红星..二氯喹啉酸抗性稗草对灰飞虱体内微生物群落结构及功能的影响[J].浙江大学学报（农业与生命科学版）,2025,51(2):277-290,14.基金项目
国家重点研发计划项目(2023YFD1400800) （2023YFD1400800）
浙江省"三农九方"科技协作计划项目(2025SNJF046,2023SNJF006). （2025SNJF046,2023SNJF006）
