中南民族大学学报（自然科学版）2025，Vol.44Issue(4)：490-496,7.DOI:10.20056/j.cnki.ZNMDZK.20250408
胡椒碱对CCl4诱导小鼠急性肝损伤的保护作用
Protective effect of piperine on acute liver injury induced by CCl4 in mice
摘要
Abstract
The protective effect of piperine(PIP)on acute liver injury caused by carbon tetrachloride(CCl4)and the potential molecular mechanisms were explored.Forty male Kunming mice were stochastically divided into the normal control(NC)group,model(CCl4)group,positive control group treated with silymarin(SIY),low dose of PIP group(10 mg/kg CCl4+PIP)and high dose of PIP group(20 mg/kg CCl4+PIP).Pathological changes in livers,Liver index and liver function indices of all the mice were detected.While the activities or levels of SOD,GSH-Px,MDA,TNF-α,IL-1β,and IL-6 in the liver were respectively evaluated by relevant kit assays;Gene transcript levels and protein expression of NLRP3,ASC,Caspase-1 and IL-1β in the liver were measured using quantitative real-time PCR,Western blot,and immunohistochemistry.Results showed PIP effectively improved the indexes of liver function and pathological changes of liver tissue,alleviated the oxidative stress state.Further research showed that PIP reduced the mRNA and the protein expressions of NLRP3,ASC,caspase-1 and IL-1β.In conclusion,PIP may play a protective role in CCl4-induced acute liver injury by alleviating oxidative stress and inhibiting inflammatory reactions.关键词
胡椒碱/四氯化碳/NLRP3炎症小体/氧化应激/炎症反应Key words
piperine/CCl4/NLRP3 inflammasome/oxidative stress/inflammatory reaction分类
药学引用本文复制引用
邓旭坤,王绰,郑珊珊,余惠凡,黎炎梅,舒广文..胡椒碱对CCl4诱导小鼠急性肝损伤的保护作用[J].中南民族大学学报（自然科学版）,2025,44(4):490-496,7.基金项目
湖北省自然科学基金资助项目(2025AFD508) （2025AFD508）
江西赣江海智B类人才计划(GHZ22018) （GHZ22018）
武当地方中药研究湖北省重点实验室(湖北医药学院,WDCM2023012)开放项目 （湖北医药学院,WDCM2023012）
江西省"双千计划"科技创新高端人才(青年)项目(JXSQ2019201105) （青年）
