植物遗传资源学报2025，Vol.26Issue(4)：797-807,11.DOI:10.13430/j.cnki.jpgr.20240822001
小麦穗部性状近等基因系的创制及穗粒数候选基因分析
Creation of Near-Isogenic Lines and Analysis of Candidate Genes for Grain Number Per Spike in Wheat
摘要
Abstract
To develop novel genetic materials with breeding potential and identify genetic intervals regulating grain number per spike in wheat,we conducted a comprehensive study using near-isogenic lines(NILs)through crossing the octoploid Thinopyrum ponticum derived wheat cultivars Hengguan 35 and Kenong 199.Seven yield-related traits,including plant height,effective tiller number,spike length,spikelet number per spike,grain number per spike,grain yield per plant and thousand grain weight,were systematically evaluated.Genome-wide scanning was performed using the 660K SNP array to identify polymorphic loci and conserved physical intervals between two pairs of NILs.Candidate genes were predicted through integrated analysis of gene annotation and expression profiles within the candidate regions.The results indicated that NIL pairs N81/N82 and N86/N87 exhibited significant differences in spike-related traits while maintaining genetic similarities of 98.02％and 98.78％,respectively.SNP polymorphism analysis identified three conserved genomic regions associated with spike architecture,662-669 Mb on chromosome 1B,19-25 Mb on chromosome 3B,and 541-548 Mb on chromosome 5B.Through integration of QTL mapping data,gene functional annotation,expression analysis,and orthologous gene comparison,we identified three putative candidate genes regulating grain number per spike:TraesCSlB02G443200,encoding malate dehydrogenase on chromosome 1B,TraesCS3B02G042400,encoding an AP2/ERF transcription factor on chromosome 3B,and TraesCS5B02G366500,encoding a C2H2-type zinc finger protein on chromosome 5B.These findings provide a theoretical reference for identifying genes regulating grain number per spike in wheat.关键词
小麦/660K SNP芯片/近等基因系/穗部相关性状/候选基因Key words
wheat/660K SNP array/near-isogenic lines/spike related traits/candidate genes引用本文复制引用
陈旺,王殿,宋波,刘易科,朱展望,卫波,宁强..小麦穗部性状近等基因系的创制及穗粒数候选基因分析[J].植物遗传资源学报,2025,26(4):797-807,11.基金项目
国家自然科学基金(32072061,32272173,31571750)National Natural Science Foundation of China(32072061,32272173,31571750) （32072061,32272173,31571750）
