作物杂志Issue(2)：29-39,11.DOI:10.16035/j.issn.1001-7283.2025.02.005
甘蓝型油菜LPAT家族基因鉴定与表达模式分析
Identification and Expression Pattern Analysis of LPAT Family Genes in Brassica napus
摘要
Abstract
Lysophosphatidylcholine acyltransferase(LPAT)plays a crucial role in the accumulation and metabolism of plant fatty acids.To investigate the evolution and expression pattern of the LPAT gene family in Brassica napus,we identified BnLPAT genes in the B.napus genome using bioinformatics methods.The physical and chemical properties,phylogeny,gene structure,cis-elements,and expression patterns of BnLPAT genes were analyzed.The results identified 44 LPAT genes in the B.napus genome,which were unevenly distributed on 17 chromosomes.Based on phylogenetic relationships,they were classified into five branches,LPAT1 to LPAT5,respectively.Members of adjacent branches had shared similar gene structures and conserved motifs.The 42 types of cis-elements were identified in the putative BnLPAT gene promoter regions,including four categories.Furthermore,expression pattern analysis revealed that BnLPAT genes were detected in different tissue parts.We further analyzed the expression characteristics of 12 BnLPAT genes during seed development and four genes in varieties with high,medium,and low oil contents.关键词
溶血磷脂酰胆碱酰基转移酶(LPAT)/甘蓝型油菜/基因家族鉴定/表达模式Key words
Lysophosphatidylcholine acyltransferase(LPAT)/Brassica napus/Identification of gene family/Expression pattern引用本文复制引用
李坤杰,刘念,丁磊,朱燕,蒙大庆,范其新,李迎春,陈军..甘蓝型油菜LPAT家族基因鉴定与表达模式分析[J].作物杂志,2025,(2):29-39,11.基金项目
四川省重大科技专项(2022ZDZX0015) （2022ZDZX0015）
四川省创新团队项目(sccxtd-2023-03) （sccxtd-2023-03）
国家油菜产业技术体系建设项目(CARS-12) （CARS-12）
重庆市技术创新与应用发展专项(cstc2021jscx-cylhX0003) （cstc2021jscx-cylhX0003）
四川省"十四五"育种攻关项目(2021YFYZ0018) （2021YFYZ0018）
四川省"天府菜油"行动奖补项目(2023TFRO06) （2023TFRO06）
