作物杂志Issue(2)：47-53,7.DOI:10.16035/j.issn.1001-7283.2025.02.007
基于SSR标记对69份玉米种质资源的遗传多样性分析
Genetic Diversity Analysis of 69 Maize Germplasm Resources Based on SSR Markers
摘要
Abstract
In order to broaden the genetic basis of maize inbred lines in Hebei province and accelerate the development and utilization of excellent germplasms,SSR markers were used to analyze genetic diversity of 39 inbred lines and 30 varieties.The results showed that a total of 97 alleles were amplified from 32 polymorphic SSR markers,with an average of three alleles amplified per primer pair.The percentage of polymorphic loci was 68.75%.The average number of effective alleles,Shannon information index,expected heterozygosity,and PIC values of the primers were 1.40,0.31,0.20,and 0.27,respectively.The genetic distance of the maize population ranged from 0.103 to 0.844,and the inbred lines had the closest genetic exchange with varieties from Hebei province and Beijing,with relatively close genetic relationships and smaller genetic distances of 0.140 and 0.155,respectively,showing a certain degree of correlation between genetic distance and geographical distance.The test materials were divided to five groups by cluster analysis,and the inbred lines were clearly distinguished;The varieties from the three northeast provinces of China,Gansu,and some parts of Hebei and Beijing were grouped together,and most of the cultivated crops in the region mature once a year.The eight early maturing varieties were closely related,indicating that SSR markers could to some extent distinguish germplasm types.The genetic diversity and purity of inbred lines are the highest,which can be further selected and utilized.关键词
SSR标记/玉米/种质资源/遗传多样性Key words
SSR marker/Maize/Germplasm resource/Genetic diversity引用本文复制引用
常宏兵,王晨,何美敬,曹熙敏,俞凤芳,曹晓良,宋炜,吕爱枝..基于SSR标记对69份玉米种质资源的遗传多样性分析[J].作物杂志,2025,(2):47-53,7.基金项目
河北省重点研发计划项目现代种业科技创新专项(21326319D,22326317D) （21326319D,22326317D）
河北省教育厅基本科研业务费(JYT2023010) （JYT2023010）
