作物杂志Issue(2)：54-65,12.DOI:10.16035/j.issn.1001-7283.2025.02.008
桔梗白花和紫花发育过程基因差异表达研究
Study on Differential Gene Expression during the Development of White and Purple Flowers of Platycodon grandiflorus
摘要
Abstract
To explore the genes and pathways influencing the color formation of Platycodon grandiflorus purple flowers,the BMKCloud platform from Biomarker Technologies was used to conduct transcriptome sequencing on both white and purple P.grandiflorus flowers at three stages(bud,color changing,and blooming).The differentially expressed genes related to anthocyanin metabolism in P.grandiflorus were screened.Five KEGG pathways related to anthocyanin metabolism were screened out.A total of 11 candidate genes involved in anthocyanin biosynthesis were screened out,including nine key enzyme genes(HCT,CHI,DFR,F3'5'H,ANS,3'GT,CCR,NAR,HPPR)and two glycosylase genes(β-glucosidase BoGH3B and UGT79B6),and their continuous expression affects the formation of purple flowers of P.grandiflorus.This research constructed an expression regulation pathway for the formation of P.grandiflorus purple flowers and identified the candidate genes involved in this process,thus providing new insights for the genetic and molecular mechanism research of P.grandiflorus purple flowers.关键词
转录组/花青素/桔梗/花色/差异表达基因Key words
Transcriptome/Anthocyanins/Platycodon grandiflorus/Flower color/Differently expressed genes引用本文复制引用
齐书娅,严一字,金卓,吴松权..桔梗白花和紫花发育过程基因差异表达研究[J].作物杂志,2025,(2):54-65,12.基金项目
吉林省科技厅重点研发项目"高品质道地药材桔梗的生态种植技术集成"(20220204048YY) （20220204048YY）
