中药新药与临床药理2025，Vol.36Issue(4)：534-541,8.DOI:10.19378/j.issn.1003-9783.2025.04.006
基于"肝病实脾"理论探讨白术多糖对酒精性肝损伤大鼠的保护作用
Study on the Protective Effect of Polysaccharide from Atractylodes on Alcoholic Liver Injury in Rats Based on the Theory of"Treating the Liver by Reinforcing the Spleen"
摘要
Abstract
Objective To investigate the protective effect of polysaccharide from atractylodes on alcoholic liver injury(ALI)in rats based on the theory of"treating the liver by reinforcing the spleen".Methods The rats were randomly divided into model group,polysaccharide from atractylodes low-dose and high-dose group,polyene phosphatidylcholine group,polysaccharide from atractylodes high-dose+trimethylamine oxide group and control group,with 12 rats in each group.Except for the control group,the model was replicated by intragastric administration of liquor for 6 consecutive weeks.The model was replicated while drug intervention was administered once a day for 6 consecutive weeks.The changes in serum alanine transaminase(ALT)and aspartate aminotransferase(AST)levels in rats were detected;HE staining was applied to detect pathological changes in rat liver and spleen tissues;the spleen index of rats was detected;flow cytometry was applied to detect the expressions of CD4+,CD8+,and CD4+/CD8+in splenic tissue;the changes in malondialdehyde(MDA),superoxide dismutase(SOD)contents,and reactive oxygen species(ROS)fluorescence intensity in rat spleen tissue were detected;Western Blot detect glucose regulated protein 78(GRP78),cysteinyl aspartate specific protease 12(Caspase-12)proteins in spleen and liver tissues.Results Compared with the control group,the model group had severe pathological damage to the liver and spleen tissues of rats,the levels of AST and ALT in serum,spleen index,CD8+level in spleen tissue,MDA content,ROS fluorescence intensity,and the protein expressions of GRP78 and Caspase-12 in spleen and liver tissues elevated,the levels of CD4+,CD4+/CD8+,and SOD content in spleen tissue decreased(P＜0.05);compared with the model group,the pathological damage to the liver and spleen tissues of rats in the polysaccharide from atractylodes low-dose group,polysaccharide from atractylodes high-dose group and polyene phosphatidylcholine group was alleviated,the levels of AST and ALT in serum,spleen index,CD8+level in spleen tissue,MDA content,ROS fluorescence intensity,and the protein expressions of GRP78 and Caspase-12 in spleen and liver tissues reduced,the levels of CD4+,CD4+/CD8+,and SOD content in spleen tissue increased(P＜0.05);trimethylamine oxide weakened the inhibitory effect of high-dose polysaccharide from atractylodes on oxidative stress in the spleen of ALI rats and its improvement on immune function.Conclusion Polysaccharide from atractylodes may inhibit spleen oxidative stress and improve immune function in ALI rats by inhibiting ROS/GRP78/Caspase-12 signaling pathway.关键词
白术多糖/活性氧/葡萄糖调节蛋白78/半胱氨酸天冬氨酸蛋白酶12通路/酒精性肝损伤/肝病实脾/氧化应激/免疫功能/大鼠Key words
Polysaccharide from atractylodes/reactive oxygen species/glucose regulated protein 78/cysteinyl aspartate specific protease 12 pathway/alcoholic liver injury/treating the liver by reinforcing the spleen/oxidative stress/immune/rats分类
医药卫生引用本文复制引用
左晓利,娄静,闫悦..基于"肝病实脾"理论探讨白术多糖对酒精性肝损伤大鼠的保护作用[J].中药新药与临床药理,2025,36(4):534-541,8.基金项目
河南省中医药科学研究专项课题(2022ZY1147). （2022ZY1147）
