中药新药与临床药理2025，Vol.36Issue(4)：542-551,10.DOI:10.19378/j.issn.1003-9783.2025.04.007
苇芩泻白汤在急性肺损伤中的分子机制研究
Study on the Molecular Mechanism of Weiqin Xiebai Decoction in Acute Lung Injury
摘要
Abstract
Objective To explore the therapeutic mechanism of Weiqin Xiebai Decoction on acute lung injury(ALI)based on network pharmacology and in vivo experiments.Methods(1)TCMSP database was used to screen the active components of Weiqin Xiebai Decoction and its potential target information,and disease targets related to acute lung injury were obtained in OMIM,TTD and GeneCards databases.Cytoscape 3.10 software was used to construct the network of"Chinese medicines-active ingredients-common targets",and the main active ingredients were analyzed.The protein-protein interaction(PPI)network of common targets was established with the help of STRING database.In addition,GO function enrichment and KEGG pathway enrichment analysis were performed on the common targets.(2)The mouse model of acute lung injury was established by intratracheal injection of lipopolysaccharide and Weiqin Xiebai Decoction was used to intervene.The pathological changes of lung tissue were observed by HE staining.The protein and mRNA expression levels of TNF-α,IFN-γ and IL-4 in tissues were detected by immunohistochemistry and RT-qPCR,respectively.Results(1)A total of 161 active components,207 potential targets and 1 523 targets related to acute lung injury were identified in Weiqin Xiebai Decoction,and 119 common targets were obtained by intersection.Among them,17 core targets included IL-2,IL-4,IL-10,IL-6,TNF-α,IL-1β and IFN-γ.The main active components were quercetin,kaempferol,stigmasterol,β-sitosterol and acacetin.KEGG pathway enrichment analysis suggested that it mainly involved PI3K-Akt,MAPK,IL-17,Toll and Th1/Th2 signaling pathways.(2)The results of HE staining showed that Weiqin Xiebai Decoction could significantly reduce the HE score of mice with acute lung injury(P＜0.05).After treatment,the protein and mRNA expression levels of TNF-α and IFN-γ in the high-dose group were significantly decreased(P＜0.05,P＜0.01,P＜0.000 1),while the protein and mRNA expression levels of IL-4 were significantly increased(P＜0.01).Conclusion Weiqin Xiebai Decoction acts on acute lung injury through multi-component,multi-target and multi-pathway.The results of animal experiments suggest that acute lung injury is accompanied by Th1/Th2 imbalance,and Weiqin Xiebai Decoction can correct this imbalance.关键词
苇芩泻白汤/急性肺损伤/网络药理学/Th1/Th2平衡/抗炎/小鼠Key words
Weiqin Xiebai Decoction/acute lung injury/network pharmacology/Th1/Th2 balance/anti-inflammation/mice分类
医药卫生引用本文复制引用
蒋紫云,袁庆霖,陈余姗,谢燕小,王凯,黄海阳,孙志佳,钱旭胜,连乐燊..苇芩泻白汤在急性肺损伤中的分子机制研究[J].中药新药与临床药理,2025,36(4):542-551,10.基金项目
国家自然科学基金项目(82274381) （82274381）
国家中医药传承创新科研专项项目(2022QN24) （2022QN24）
东莞市临床重点专科建设项目(东卫函[2021]7号). （东卫函[2021]7号）
