中国现代中药2025，Vol.27Issue(4)：647-660,14.DOI:10.13313/j.issn.1673-4890.20240702004
基于超高效液相色谱串联四极杆静电场轨道阱质谱法的天麻眩晕宁颗粒化学成分鉴定及归属
Identification and Attribution of Chemical Components in Tianma Xuanyunning Granules Based on UPLC-Q-Exactive Orbitrap MS
摘要
Abstract
Objective:The ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbital trap mass spectrometry(UPLC-Q-Exactive Orbitrap MS)technology was used to systematically analyze and classify the chemical basis of Tianma Xuanyunning Granules and explore the fragmentation patterns of its chemical components.Methods:Waters ACQUITY UPLC HSS T3 chromatography column(100 mm×2.1 mm,1.8 μm)was used;the mobile phase was 0.1%formic acid aqueous solution(A)-acetonitrile solution(B)for elution gradient(0-20.0 min,5%-30%B;20.0-23.0 min,30%-40%B;23.0-26.0 min,40%-70%B;26.0-27.0 min,70%-90%B;27.0-30.0 min,90%B;30.0-30.5 min,90%-5%B;30.5-35.0 min,5%B).The volume flow rate was 0.3 mL·min-1,and the column temperature was 30℃.The injection volume was 2 µL,and mass spectrometry scanning was performed in positive and negative ion modes.By using Xcalibur 4.2 software,precise mass-to-charge ratio,secondary mass spectrometry fragmentation,and other information were obtained and combined with a comparison of reference materials and literature data for compound analysis,so as to analyze its fragmentation patterns.Results:A total of 180 compounds were identified,including 53 flavonoids,35 terpenoids,29 nitrogen-containing compounds,25 organic acids,18 phenols,7 phthalides,and 13 other components.All 180 compounds were classified,with the top three medicinal herbs being Radix Paeoniae Alba,Radix et Rhizoma Glycyrrhizae,and Rhizoma Gastrodiae.The corresponding compounds were 50,49,and 26,respectively.Conclusion:The established method can quickly and accurately identify the chemical components in Tianma Xuanyunning Granules,providing a basis for the basic research of Tianma Xuanyunning Granules and a reference for quality control.关键词
天麻眩晕宁颗粒/超高效液相色谱串联四极杆静电场轨道阱质谱法/化学成分/成分归属Key words
Tianma Xuanyunning Granules/UPLC-Q-Exactive Orbitrap MS/chemical composition/component attribution分类
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吴健,关永霞,范建伟,张贵民..基于超高效液相色谱串联四极杆静电场轨道阱质谱法的天麻眩晕宁颗粒化学成分鉴定及归属[J].中国现代中药,2025,27(4):647-660,14.基金项目
山东省重点研发计划(重大科技创新工程)项目(2021CXGC010508) （重大科技创新工程）
