中国现代中药2025，Vol.27Issue(4)：667-673,7.DOI:10.13313/j.issn.1673-4890.20240912003
短果大叶芹根部水提取物的化学成分及其抗氧化效果
Chemical Components and Antioxidant Effects of Water Extract from the Roots of Spuriopimpinella brachycarpa
摘要
Abstract
Objective:To study the antioxidant capacity of the water extract from the roots of Spuriopimpinella brachycarpa(Kom.)Kitag.and its effect on the lifespan of Caenorhabditis elegans,and to explore its medicinal potential.Methods:The chemical components in the water extract from the roots of S.brachycarpa were identified using ultra-high-performance liquid chromatography coupled with quadrupole Orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap-MS).Wild-type and transgenic SOD-3::GFP C.elegans were used to evaluate the antioxidant effects of the water extract from S.brachycarpa roots.An oxidative stress model was induced by 100 mmol·L-1 paraquat,and different concentrations(125-500 mg·L-1)of the extract were administered to L1 stage C.elegans.The effects on antioxidant enzyme activity,reactive oxygen species(ROS)levels,and lifespan were evaluated.Results:Chemical analysis revealed that the water extract from the roots of S.brachycarpa contained phenolic acids such as chlorogenic acid,caffeic acid,and quinic acid,which exhibited significant antioxidant activity.The extract significantly reduced the production of ROS in the C.elegans,restoring them to normal levels,and rescued the decline in SOD-3::GFP expression caused by oxidative stress,thereby maintaining the activity of superoxide dismutase(SOD).The water extract also delayed the death of C.elegans induced by paraquat,showing strong antioxidant effects and prolonging lifespan.Conclusion:The water extract from the roots of S.brachycarpa enhances the antioxidant defense system of C.elegans,reduces oxidative stress,and prolongs lifespan,providing a scientific basis for its application as a natural antioxidant.关键词
短果大叶芹/根部水提取物/抗氧化/秀丽隐杆线虫/寿命延长/酚酸类化合物Key words
Spuriopimpinella brachycarpa(Kom.)Kitag./water extract from roots/antioxidation/Caenorhabditis elegans/life prolonging/phenolic acid compounds分类
医药卫生引用本文复制引用
乔燕,韩君,袁强华..短果大叶芹根部水提取物的化学成分及其抗氧化效果[J].中国现代中药,2025,27(4):667-673,7.基金项目
成都中医药大学附属医院科研能力提升"百人计划"优秀人才项目(22-Y31) （22-Y31）
