中国现代中药2025，Vol.27Issue(4)：674-684,中插1-中插5,16.DOI:10.13313/j.issn.1673-4890.20241105005
基于TPP-MS高通量靶点筛选技术的小青龙汤改善呼吸道疾病作用机制及潜在新功效研究
Mechanism of Action and Potential New Therapeutic Effects of Xiaoqinglong Decoction in Improving Respiratory Diseases Based on TPP-MS High-throughput Target Screening Technology
摘要
Abstract
Objective:By applying a targeted research method of classical prescriptions of traditional Chinese medicine(TCM)based on the research system of"classical prescriptions of TCM,high-throughput target screening,target association,and new clinical application",the mechanism of action of Xiaoqinglong Decoction(XQLD)in the treatment of pulmonary diseases was clarified,and its potential new therapeutic effects were explored.Methods:The technique of thermal proteome profiling(TPP)-high-resolution mass spectrometry(MS)was employed to perform high-throughput screening on the comprehensive targets of XQLD in rat lung tissue.The ingenuity pathway analysis(IPA)database was utilized to investigate the association between the targets of XQLD and downstream regulatory proteins.Furthermore,the Genevestigator platform was used for further exploration and analysis of target-associated diseases.Results:A total of 12 direct and 14 indirect targets of XQLD were screened.Through the combined analysis of the IPA database and the Genevestigator platform on the diseases associated with the targets of XQLD,it was found that these targets were involved in the treatment of bronchial asthma and chronic obstructive pulmonary disease and might treat allergic diseases and lung cancer through new anti-allergy and anti-tumor effects.Conclusion:This study clarifies the mechanism of action of XQLD in the treatment of pulmonary diseases from a target perspective and reveals its potential new therapeutic effects,including anti-allergic and anti-tumor properties.关键词
中药靶标/小青龙汤/热蛋白质组学/作用机制/肺病Key words
target of traditional Chinese medicine/Xiaoqinglong Decoction/thermal proteome profiling/mechanism of action/pulmonary disease分类
医药卫生引用本文复制引用
傅钰,邓欣祺,陈恒,黄智兴,饶沁玲,顾新阳,赵乃轩,王春国,孟闫燕..基于TPP-MS高通量靶点筛选技术的小青龙汤改善呼吸道疾病作用机制及潜在新功效研究[J].中国现代中药,2025,27(4):674-684,中插1-中插5,16.基金项目
国家自然科学基金项目(82004167) （82004167）
第六批北京市级中医药专家学术经验师承项目(京中医科字[2021]169号) （京中医科字[2021]169号）
北京中医药大学实验技术(操作规程)标准化研究项目(2023-syjs-01) （操作规程）
