中国现代中药2025，Vol.27Issue(4)：710-717,中插8,9.DOI:10.13313/j.issn.1673-4890.20250205003
基于非靶向代谢组学的光果甘草和胀果甘草代谢物分析
Metabolite Analysis of Glycyrrhiza glabra and Glycyrrhiza inflata Based on Untargeted Metabolomics
摘要
Abstract
Objective:Differential metabolite analyses of two medicinal licorice plants,Glycyrrhiza glabra and G.inflata,were carried out on the medicinal parts to provide a reference for licorice species selection and cultivation.Methods:Ultra performance liquid chromatography-mass spectrometry(UPLC-MS)combined with principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),and heatmap clustering were used to identify and analyze the metabolites in the two licorice plants;the Kyoto Encyclopedia of Genes and Genomes(KEGG)database was used to annotate the differential metabolites and perform metabolic pathway and enrichment analysis.Results:By comparing the databases,a total of 1 512 metabolites were identified,including terpenoids,flavonoids,alkaloids,etc.There were 167 differential metabolites,105 up-regulated metabolites,and 62 down-regulated metabolites in G.glabra compared with G.inflata.Among them,44 flavonoid differential components,as well as 14 flavonoids such as liquiritigenin and isopongaflavone were significantly accumulated in G.glabra,and 31 flavonoids such as 6,8-diprenylgenistein and saponarin were significantly accumulated in G.inflata.The metabolic pathway enrichment analysis of the differential metabolites indicated that they were closely related to flavonoid biosynthesis,phenylpropanoid biosynthesis,etc.Conclusion:The paper clarifies the types and relative contents of metabolites in G.glabra and G.inflata,providing a reference for enriching the metabolite library and guiding variety selection and breeding of licorice species.关键词
光果甘草/胀果甘草/代谢组学/黄酮类Key words
Glycyrrhiza glabra L./G.inflata Bat./metabolomics/flavonoids分类
中医学引用本文复制引用
赵玉洋,张逸风,徐瑶,吴晓毅,姜丹,刘春生..基于非靶向代谢组学的光果甘草和胀果甘草代谢物分析[J].中国现代中药,2025,27(4):710-717,中插8,9.基金项目
中华中医药学会(2022-2024年度)青年人才托举工程项目(CACM-2022-QNRC2-B23) （2022-2024年度）
