中国现代中药2025，Vol.27Issue(4)：718-728,11.DOI:10.13313/j.issn.1673-4890.20240913005
基于网络药理学的栀子与水栀子比较分析
Comparative Analysis of Gardenia jasminoides Ellis and G.jasminoides var.radicans Makino Based on Network Pharmacology
摘要
Abstract
Objective:This study aims to compare the composition and mechanisms of Gardenia jasminoides Ellis and G.jasminoides var.radicans Makino through network pharmacology to comprehensively explore the medicinal potential of G.jasminoides radicans.Methods:The main active constituents of G.jasminoides and G.jasminoides var.radicans were screened via TCMSP and SwissADME through network pharmacology,with target predicted through STRING database.In addition,the potential mechanism of it in treating jaundice and hepatitis was analyzed through gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).Molecular docking was used to compare the binding affinities of major constituents with related targets.Additionally,in an LPS-induced RAW264.7 cell inflammation model,after different concentrations of geniposide,genipin,crocin,crocin Ⅱ and crocetin were added,CCK-8 was used to assess cell viability,NO production was detected by Griess,and TNF-α,IL-6,and IL-1β levels were tested by ELISA to evaluate anti-inflammatory effects.Results:G.jasminoides Ellis and G.jasminoides var.radicans Makino exhibited multiple common targets for jaundice and hepatitis,with GO and KEGG analyses revealing high similarities and associations with inflammation-related pathways.Five shared compounds geniposide,genipin,crocin,crocin,and crocetin identified by molecular docking and toxicity prediction showed significant pro-proliferative effects on LPS-induced RAW264.7 cells and notable inhibition of the inflammatory factors NO,TNF-α,IL-6,and IL-1β.Conclusion:Comparative analysis through network pharmacology reveals that G.jasminoides Ellis and G.jasminoides var.radicans Makino share similar constituents and mechanisms in treating jaundice and hepatitis.Among them,shared constituents geniposide,genipin,crocin,crocin Ⅱ,and crocetin demonstrate significant therapeutic potential.These findings provide new perspectives and reference for the development of G.jasminoides var.radicans Makino.关键词
栀子/水栀子/网络药理学/分子对接/抗炎活性Key words
Gardenia jasminoides Ellis/G.jasminoides var.radicans Makino/network pharmacology/molecular docking/anti-inflammatory activity分类
中医学引用本文复制引用
侯子雨,孙乐,曾铁鑫,黄佳丽,许利嘉,刘海波,董政起..基于网络药理学的栀子与水栀子比较分析[J].中国现代中药,2025,27(4):718-728,11.基金项目
中国医学科学院医学与健康科技创新项目(2022-I2M-1-018) （2022-I2M-1-018）
