中国现代中药2025，Vol.27Issue(4)：729-737,9.DOI:10.13313/j.issn.1673-4890.20240515002
破壁法提取淫羊藿的工艺研究与优化
Optimization of Cell Wall Disruption Technology for Extraction of Epimedii Folium
摘要
Abstract
Objective:To investigate the optimal extraction technology of Epimedii Folium.Methods:The cell wall disruption method was selected for extraction by comparing it with the reflux extraction and ultrasonic-assisted extraction.Based on the single-factor experiment,the cell wall disruption time,ethanol concentration,and solid-to-liquid ratio were selected as the influencing factors.The high-performance liquid chromatography(HPLC)was used to determine the contents of epimedin A1,epimedin A,epimedin B,epimedin C,and icariin.The total extraction amount was used as the evaluation index.The Box-Behnken response surface method was used to optimize the extraction technology.The state of the liquid extracted by the reflux method,ultrasonic method,and cell wall disruption method was observed using a microscope.Results:The cell wall disruption method had the best extraction effect on Epimedii Folium;in a single-factor experiment of cell wall disruption time,the maximum total extraction amount was 26.54 mg·g-1,which was approximately 28.46%and 21.80%higher than that of the reflux method and ultrasound method,respectively.The optimal extraction conditions for Box-Behnken response surface method optimization were:cell wall disruption time of 4 min,ethanol concentration of 50%,and solid-to-liquid ratio of 30:1.Under these conditions,the total extraction amount was 32.68 mg·g-1;the total transfer rate was 94.72%,and the RSD value was 1.59%.The optimized process was stable and feasible.The microscopic observations showed that the tissue of Epimedii Folium leaves was more fully exposed to solvent,and the extraction efficiency was improved by using the cell wall disruption method.Conclusion:The cell wall disruption method had a higher efficiency for extracting Epimedii Folium.It could save time and energy and improve the transfer rate,which has a guiding effect on the extraction and production process of Epimedii Folium.关键词
淫羊藿/破壁/淫羊藿苷/朝藿定/高效液相色谱法/Box-Behnken响应面法Key words
Epimedii Folium/cell wall disruption/icariin/epimedin/HPLC/Box-Behnken response surface method分类
中医学引用本文复制引用
陈红斌,洪伦波,陶益,杨丽钗,吴晓慧,徐建兵,汪娟..破壁法提取淫羊藿的工艺研究与优化[J].中国现代中药,2025,27(4):729-737,9.基金项目
浙江省自然科学基金项目(LY21H280008) （LY21H280008）
