中国现代中药2025，Vol.27Issue(4)：742-749,8.DOI:10.13313/j.issn.1673-4890.20240530009
三七粉及三七伤药片质量评价方法研究
Quality Evaluation Method of Notoginseng Radix et Rhizoma Powder and Sanqi Shangyao Tablets
摘要
Abstract
Objective:To establish a quality evaluation method for Notoginseng Radix et Rhizoma powder and Sanqi Shangyao Tablets.Methods:Microscopic observation,thin-layer chromatography(TLC),ultra-high performance liquid chromatography(UPLC),and UPLC-tandem mass spectrometry(MS/MS)were used for qualitative and quantitative analyses of saponins in Notoginseng Radix et Rhizoma powder and Sanqi Shangyao Tablets.Results:The TLC method for identification and determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,and ginsenoside Rd in Notoginseng Radix et Rhizoma powder and Sanqi Shangyao Tablets was established.The established method showed clear spots with good separation in the identification and favorable linear relationships in the determination of key components.The recovery of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,and ginsenoside Rd in Notoginseng Radix et Rhizoma powder was 99.0%,99.3%,100.6%,101.5%,and 98.8%,with the relative standard deviation(RSD)of 1.4%,1.3%,1.6%,0.7%,and 1.6%,respectively.The recovery of above-mentioned components in Sanqi Shangyao tablets was 95.5%,99.8%,102.0%,98.5%,and 97.0%,with the RSD of 7.3%,2.9%,3.8%,3.3%,and 4.1%,respectively.Conclusion:The established method demonstrated high specificity and reproducibility and can be used for the quality evaluation of Notoginseng Radix et Rhizoma powder and Sanqi Shangyao Tablets.关键词
三七/三七粉/薄层鉴别/超高效液相色谱法/超高效液相色谱-质谱法Key words
Notoginseng Radix et Rhizoma/Notoginseng Radix et Rhizoma powder/TLC/UPLC/UPLC-MS/MS分类
中医学引用本文复制引用
赵振霞,耿莲,安丽康,孔亚萍,刘永利..三七粉及三七伤药片质量评价方法研究[J].中国现代中药,2025,27(4):742-749,8.基金项目
河北特色中药饮片炮制规范化研究及应用示范项目(22372503D) （22372503D）
