中国现代中药2025，Vol.27Issue(4)：750-755,6.DOI:10.13313/j.issn.1673-4890.20240629001
基于GC-MS分析止咳桃花散中的挥发性成分
Volatile Components in Zhike Taohua Powder by Gas Chromatography-Mass Spectrometry
摘要
Abstract
Objective:Gas chromatography-mass spectrometry(GC-MS)was used to establish a fingerprint of Zhike Taohua Powder and determine the content of volatile components to evaluate the quality of volatile components in Zhike Taohua Powder,providing a basis for evaluating the quality of volatile components in Zhike Taohua Powder and improving the preparation.Method:The analysis was performed on an HP-5MS capillary column,taking helium as the carrier gas at a flow rate of 1.0 mL·min-1 with a 25:1 split ratio and a 1.0 μL injection volume.The detector was a mass spectrometer with a detector temperature of 250℃and an inlet temperature of 250℃.The column temperature procedure was as follows:initial temperature at 82 ℃(held for 20 min),increased at 10℃·min-1 to 205 ℃(held for 7 min),then ramped at 20 ℃·min-1 to 250 ℃ without holding.Results:There were sixteen common peaks and eleven core peaks,with similarities of more than 0.99.The concentration ranges of isoborneol,L-borneol,menthol,and muscone were 1.073 0-21.460 0,0.998 0-19.960 0,0.069 5-1.390 0,0.017 0-0.340 0 μg·mL-1(r=0.999 9),respectively,with good linear relationships.Conclusion:The proposed method for determining volatile components in Zhike Taohua Powder is easy to implement with high sensitivity and reliable results,providing a reference for the quality evaluation of traditional Chinese medicine.关键词
止咳桃花散/气相色谱-质谱法/挥发性成分/指纹图谱/含量测定Key words
Zhike Taohua Powder/GC-MS/volatile component/fingerprints/content determination分类
中医学引用本文复制引用
王媚,薛蕙,冯李乐,瞿钟欢,崔春利,张小飞,郭东艳,史亚军..基于GC-MS分析止咳桃花散中的挥发性成分[J].中国现代中药,2025,27(4):750-755,6.基金项目
陕西省科技厅项目(2023-YBSF-474) （2023-YBSF-474）
陕西省中医药管理局中药制药工程重点学科资助项目(2017001) （2017001）
陕西省中药制药重点学科资助项目(303061107) （303061107）
