遵义医科大学学报2025，Vol.48Issue(4)：325-331,7.
一株高毒力肺炎克雷伯菌噬菌体的基因组和生物学特征分析
The genomic and biological characteristics of a hypervirulent klebsiella pneu-moniae bacteriophage
摘要
Abstract
Objective To isolate and identify a hypervirulent Kp(hvKp)bacteriophage with potential clinical application value,providing a basis for the development of novel antimicrobial agents.Methods Using a hvKp isolate as the host bacterium,we isolated and characterized a bacteriophage capable of lysing this strain.The ge-nomic features,morphological characteristics,environmental stress tolerance,lysis efficiency,and host range were analyzed for the identified bacteriophage.Results Employing the hvKp isolate QD22 as the host bacterium for phage isolation,the obtained phage PTF1 was found not only to lyse QD22 but also multiple other hvKp iso-lates,with a lysis rate of up to 31.9%against hvKp isolates.Sequence type and capsular type analysis revealed that the lysis rates of PTF1 against isolates of sequence type 23 and capsule type K1 were 87.5%and 86.7%,respectively.Phage PTF1 belongs to the Podoviridae family and lacks virulence genes and antibiotic resistance genes.Biological characteristic analysis revealed that the optimal multiplicity of infection(MOI)for PTF1 is as low as 10-3,with a maximum burst size of 120 PFU per cell.Even at an MOI as low as 10-6,PTF1 can com-pletely inhibit the proliferation of the host bacterium.Environmental stress tolerance analysis indicated that PTF1 maintains high biological activity across a temperature range of 4～60℃and pH levels from 4 to 10.Conclusion Phage PTF1 is a short-tailed phage with lytic efficiency and environmental stress tolerance,capable of lysing 31.9%of hvKp isolates,indicating significant potential for clinical applications.关键词
肺炎克雷伯菌/噬菌体/高毒力/荚膜型Key words
klebsiella pneumoniae/bacteriophage/hypervirulence/capsular type分类
生物学引用本文复制引用
甘霖,田紫妍,袁静..一株高毒力肺炎克雷伯菌噬菌体的基因组和生物学特征分析[J].遵义医科大学学报,2025,48(4):325-331,7.基金项目
国家自然科学基金资助项目(NO:82402639) （NO:82402639）
北京市属医院科研培育计划项目(NO:PX2025048). （NO:PX2025048）
