遵义医科大学学报2025，Vol.48Issue(4)：347-353,7.
排卵后老化对大鼠体内卵母细胞质量及子鼠行为学的影响
Study on the effects of post ovulatory aging on the quality of oocytes in rats and the behavior of offspring
摘要
Abstract
Objective To study the effects of post ovulatory aging(POA)on the quality of oocytes and the be-havior of offspring,and to explore the optimal window period for oocyte fertilization.Methods Female SD rats were intraperitoneally injected with pregnant mare serum gonadotropin(PMSG)to promote oocyte maturation.After 48 hours,HCG was injected to promote oocyte expulsion.Subsequently,the rats were randomly divided in-to the following groups:0-hour post ovulation(AO)group,AO 6-hour group,AO 12-hour group,AO 18-hour group,and AO 24-hour group.At the end of the experiment,the fallopian tubes of some female SD rats in each group were dissected to obtain oocytes,and the number of oocytes in each group was counted respectively;the morphology of oocytes and abnormal oocytes were observed under a stereomicroscope;Mito-Tracker Red CMXRos,JC-1 and DCHF-DA fluorescent probes were used to detect the mitochondrial membrane potential and reactive oxygen species(ROS)levels of oocytes.Another set of the female SD rats was caged with male rats for fertilization until natural delivery,and the growth and development of the offspring in each group were statistically analyzed;the morris water maze was used to detect the learning and memory functions of the adult offspring in each group.Results As the ovulation time prolonged,the abnormal rates of oocytes,such as irregular morpholo-gy,widened cytoplasmic gaps,and cell rupture,gradually increased;the ROS levels gradually rose;and the mi-tochondrial membrane potential decreased.The number of offspring born in the AO 12 h group was the largest,but there was no significant difference in growth weight between male rats groups.Female rats showed differences in growth weight between some groups at 3,6,and 8 weeks(P＜0.05).The Morris water maze results of the 8-week-old offspring rats showed that the AO 12 h group had an advantage in escape latency and distance compared to other groups,and POA had a certain impact on the learning and memory function of the offspring rats.Conclusion Under natural conditions,within 12 hours after ovulation in female rats is the optimal fertilization window period,which can effectively avoid the adverse effects of POA on the fertilization rate and offspring.关键词
排卵后老化/卵母细胞/活性氧/线粒体功能/Morris水迷宫Key words
post ovulation aging/oocytes/reactive oxygen species/mitochondrial function/morris water maze分类
临床医学引用本文复制引用
杨江权,任峻青,王利娟,肖雁冰..排卵后老化对大鼠体内卵母细胞质量及子鼠行为学的影响[J].遵义医科大学学报,2025,48(4):347-353,7.基金项目
遵义市科技计划项目[NO:遵市科合HZ字(2023)357]. （2023）
