遵义医科大学学报2025，Vol.48Issue(4)：354-360,368,8.
贵州柞木原种提取液降低高尿酸血症大鼠血尿酸水平及机制
An examination was conducted of the chemical constituents and pharmacotox-icity of the Guizhou xylosma japonicum original extract
摘要
Abstract
Objective To explore the therapeutic mechanism of Guizhou xylosma japonicum original extract in treatment of hyperuricemia rats.Methods Simulation of hyperuricemia by means of animal modeling with intrap-eritoneal injection of potassium oxonate and feeding of 25%yeast paste diets,and rats were divided into blank control group,model group,model+Guizhou xylosma japonicum original extract high-dose group 6.4 g/(kg·d),medium-dose group 3.2 g/(kg·d),low-dose group 1.6 g/(kg·d)and allopurinol control group 75 mg/(kg·d).After 3 weeks of continuous gavage,the uric acid content in the urine and blood,urea nitrogen and serum creatinine levels of the rats in each group were measured.Hematoxylin-eosin(HE)staining was used to observe the effect of the extract of Guizhou xylosma japonicum original extract on the renal pathology of rats.Western blotting was employed to assess the expression levels of xanthine oxidase(XOD)protein in the liver and urate transporter 1(URAT1)protein in the kidney.The Guizhou xylosma japonicum original extract were also ex-amined for their main chemical constituents.Results LC-MS was used to analyse the chemical constituents in the original species of Guizhou xylosma japonicum,and the top ten compounds were proposed,among which di-hydrosphingosine and D-sphingosine might be related to the uric acid-lowering effect of the original species of Guizhou xylosma japonicum.Indicated that compared to the model group,the original extract of Guizhou xylosma japonicum significantly decreased urinary uric acid,blood uric acid,urea nitrogen,and blood creatinine levels in model rats(P＜0.05);HE revealed that this extract improved kidney conditions by alleviating cyst luminal ede-ma,renal tubular atrophy,and reducing glomerular volume.Additionally,Western blot analysis demonstrated that the extract significantly decreased the expression of hepatic XOD protein and kidney URAT1 protein(P＜0.05).Conclusion The mechanism of Guizhou xylosma japonicum original extract in treating hyperuricemia may be related to improving renal function and regulating the expression of hepatic XOD protein and kidney URAT1 protein.关键词
贵州柞木原种提取液/高尿酸血症/尿酸/动物实验Key words
Guizhou xylosma japonicum original extract/hyperuricemia/uric acid/animal experiment分类
中医学引用本文复制引用
田维忆,王菲,赖新,何金军..贵州柞木原种提取液降低高尿酸血症大鼠血尿酸水平及机制[J].遵义医科大学学报,2025,48(4):354-360,368,8.基金项目
贵州省科技合作计划项目[NO:黔科合LH字(2015)7789]. （2015）
