中医药学报2025，Vol.53Issue(4)：22-28,7.DOI:10.19664/j.cnki.1002-2392.250069
血府逐瘀汤对房颤模型大鼠心脏保护作用研究
Study on the Cardioprotective Effects of Xuefu Zhuyu Decoction on Atrial Fibrillation Model Rats
摘要
Abstract
Objective:To investigate the effects of Xuefu Zhuyu Decoction on electrocardiogram changes,inflammatory response,oxidative stress,and other indicators in atrial fibrillation(AF)model rats,and to explore its therapeutic effects and underlying mechanisms.Methods:Sixty female rats were randomly divided into a blank group,model group,verapamil group,and high-,medium-,and low-dose Xuefu Zhuyu Decoction groups,with 10 rats in each group.Except for the blank group,AF models were established in the other groups by continuous tail vein injection of a calcium chloride(CaCl2)-acetylcholine(Ach)mixed solution for 7 days.After successful modeling,the high-,medium-,and low-dose Xuefu Zhuyu Decoction groups were administered 14.04,7.02,and 3.51 g·kg-1·d-1 of Xuefu Zhuyu Decoction,respectively,by gavage.The verapamil group was given 25 mg·kg-1·d-1 of verapamil suspension,while the blank and model groups received 10 mL·kg-1·d-1 of saline by gavage for 2 weeks,during which tail vein injection of the Ach-CaCl2 solution continued.A biological signal acquisition system was used to monitor the induction and duration of AF in rats.Enzyme-linked immunosorbent assay(ELISA)was employed to measure serum levels of tumor necrosis factor-α(TNF-α),high-sensitivity C-reactive protein(hs-CRP),reactive oxygen species(ROS),superoxide dismutase(SOD),and cardiac troponin T(cTnT).Hematoxylin-eosin(HE)staining and Masson staining were used to observe pathological changes in the left atrium of rats.Results:Rats in the model group exhibited lethargy,cyanosis,and conjunctival congestion,which significantly improved after Xuefu Zhuyu Decoction intervention.Electrocardiogram results showed that,compared with the model group,both the Xuefu Zhuyu Decoction and verapamil groups significantly prolonged AF induction time and shortened AF duration(P<0.01),with the high-dose Xuefu Zhuyu Decoction group showing the best effect.ELISA results indicated that,compared with the model group,the Xuefu Zhuyu Decoction and verapamil groups significantly reduced serum levels of hs-CRP,TNF-α,cTnT,and ROS(P<0.01)and increased SOD levels(P<0.01),with the high-dose Xuefu Zhuyu Decoction group showing the best effect.HE and Masson staining results revealed that,compared with the model group,the Xuefu Zhuyu Decoction and verapamil groups improved myocardial fiber alignment and reduced collagen fiber deposition in the interstitium,with the high-dose Xuefu Zhuyu Decoction group showing the best effect.Conclusion:Xuefu Zhuyu Decoction can improve the general condition of rats,prolong AF induction time,shorten AF duration,and alleviate myocardial injury and atrial fibrosis.Its mechanism may be related to the inhibition of excessive inflammatory factor expression and oxidative stress.关键词
血府逐瘀汤/心房颤动/心房纤维化/炎症反应/氧化应激Key words
Xuefu Zhuyu Decoction/Atrial fibrillation/Atrial fibrosis/Inflammatory response/Oxidative stress分类
医药卫生引用本文复制引用
吴春雪,修俭,冯玉华,张静怡,毕珺辉..血府逐瘀汤对房颤模型大鼠心脏保护作用研究[J].中医药学报,2025,53(4):22-28,7.基金项目
广东省中医药局科研项目(20212280) （20212280）
广东省医学科学技术研究基金项目(B2021284) （B2021284）
肇庆市第一人民医院院级科研基金项目(YJJ-2020-02-04) （YJJ-2020-02-04）
黑龙江省教育厅青年创新人才项目(15011170014) （15011170014）
黑龙江省中医药管理局科研课题项目(GY2022-03) （GY2022-03）
第七批全国老中医药专家学术经验继承项目(202276) （202276）
山西省自然科学研究面上项目(202303021211179) （202303021211179）
