中医药学报2025，Vol.53Issue(4)：38-42,5.DOI:10.19664/j.cnki.1002-2392.250071
常用铁线莲属药材总黄酮及主要活性黄酮苷元含量研究
Content Determination of Total Flavonoids and Main Active Flavonoid Aglycones in Common Clematis Medicinal Materials
摘要
Abstract
Objective:To determine the content of total flavonoids and main active flavonoid aglycones in common Clematis medicinal materials.Methods:Using six commonly used Clematis medicinal materials recorded in the 2020 edition of the Chinese Pharmacopoeia and Drug Standards of the Ministry of Health of the People's Republic of China(Clematis mandshurica,Clematis chinensis,Clematis hexapetala,Clematis armandii,Clematis tangutica,and Clematis australis),as well as collected Clematis chinensis leaves and Clematis armandii leaves as study objects.Total flavonoid content and the content of main active flavonoid aglycones(quercetin,kaempferol,luteolin,and apigenin)were determined by ultraviolet spectrophotometry and high-performance liquid chromatography(HPLC).Results:The total flavonoid content was highest in Clematis australis,followed by Clematis chinensis leaves,Clematis armandii leaves,Clematis tangutica,Clematis mandshurica,Clematis chinensis,Clematis hexapetala,and Clematis armandii.The main active flavonoid aglycones were absent in Clematis chinensis,Clematis mandshurica,Clematis hexapetala,and Clematis armandii.The content of quercetin,luteolin,and kaempferol was highest in Clematis australis,while the content of apigenin was highest in Clematis chinensis leaves.Conclusion:Significant differences were observed in the total flavonoid content and composition of main active flavonoid aglycones among commonly used Clematis medicinal materials.The Clematis medicinal materials recorded in the 2020 edition of the Chinese Pharmacopoeia(Clematis mandshurica,Clematis chinensis,Clematis hexapetala,and Clematis armandii)are not suitable as sources of flavonoid active ingredients.These findings provide a reference for the comprehensive utilization of Clematis medicinal materials and the revision of Chinese Pharmacopoeia quality standards for Clematis medicinal materials.关键词
铁线莲/总黄酮/紫外分光光度计/高效液相色谱/含量测定Key words
Clematis/Total flavonoids/Ultraviolet spectrophotometry/High-performance liquid chromatography(HPLC)/Content determination分类
中医学引用本文复制引用
吴浩震,杨俊峰,刘玉宁,尼玛次仁,朱业锦,谢和兵..常用铁线莲属药材总黄酮及主要活性黄酮苷元含量研究[J].中医药学报,2025,53(4):38-42,5.基金项目
国家自然科学基金项目(82304595) （82304595）
西藏自治区科技厅区域科技协同创新专项(QYXTZX-RKZ2022-07) （QYXTZX-RKZ2022-07）
