中医药学报2025，Vol.53Issue(4)：62-70,9.DOI:10.19664/j.cnki.1002-2392.250076
基于非靶代谢组学研究痰热腑实证型出血性中风后发生二次出血的生物标志物
Study on the Biomarkers for Secondary Hemorrhage after Hemorrhagic Stroke of Phlegm-Heat and Bowel Excess Pattern Based on Non-Targeted Metabolomics
摘要
Abstract
Objective:To explore the biomarkers for secondary hemorrhage after hemorrhagic stroke of phlegm-heat and bowel excess pattern using non-targeted metabolomics and identify biomarkers with predictive value for secondary hemorrhage,providing new reference indicators for clinical prediction of disease occurrence.Methods:Sixty patients diagnosed with hypertensive cerebral hemorrhage by Western medicine and phlegm-heat and bowel excess pattern by Chinese medicine were selected from the Department of Neurosurgery,The First Affiliated Hospital of Guangzhou University of Chinese Medicine from June 2021 to April 2023.They were divided into two groups:those who had a single hemorrhage(30 patients)and those who experienced secondary hemorrhage(30 patients).Additionally,thirty healthy individuals were recruited as controls.The UHPLC-Q-Orbitrap HRMS technique was used to identify differential metabolites in plasma between one-time hemorrhage(NCX),secondary hemorrhage(NCX+),and healthy groups(CON).Multidimensional statistical analyses,such as Principal Component Analysis(PCA),Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA),heatmap of differential metabolites,and ROC curve plotting,were employed to select potential biomarkers for predicting secondary hemorrhage.Results:There were 9 differential metabolites between the healthy group and the one-time hemorrhage group,22 between the healthy group and the secondary hemorrhage group,and 11 between the one-time and secondary hemorrhage groups.Biomarkers such as DL-methylmalonic acid,2-tert-butyl-4,6-dinitrophenol,4-heptylphenol,8-methyl-2(1H)-quinolinone,5-hydroxyindole-3-acetic acid,L-canavanine,and threonine showed area under the curve(AUC)values of 0.95,0.97,0.98,0.96,0.97,0.97,and 0.85,respectively.The tryptophan metabolism pathway had a significant difference with a p-value of 0.002 and an Impact value of 0.12;valine,leucine,and isoleucine degradation pathways showed a p-value of 0.07 and an Impact value of 0.03.Conclusion:Seven differential metabolites,DL-methylmalonic acid,2-tert-butyl-4,6-dinitrophenol,4-heptylphenol,8-methyl-2(1H)-quinolinone,5-hydroxyindole-3-acetic acid,L-canavanine,and threonine,have the potential to predict secondary hemorrhage after hemorrhagic stroke of phlegm-heat and bowel excess pattern.Tryptophan metabolism was a significantly different metabolic pathway.关键词
出血性中风/痰热腑实/二次脑出血/代谢组学Key words
Hemorrhagic stroke/Phlegm-heat and bowel excess/Secondary cerebral hemorrhage/Metabolomics分类
医药卫生引用本文复制引用
邓娟,郭凯欣,柯雪红,陈为..基于非靶代谢组学研究痰热腑实证型出血性中风后发生二次出血的生物标志物[J].中医药学报,2025,53(4):62-70,9.基金项目
国家自然科学基金项目(82074099) （82074099）
