中医药信息2025，Vol.42Issue(4)：12-18,7.DOI:10.19656/j.cnki.1002-2406.20250402
高氏盆炎方一号方对慢性盆腔炎模型大鼠抗炎作用及其对TGF-β1、MMP-2的影响
Anti-Inflammatory Effect of Gao's Pelvic Inflammation Formula No.1 on Chronic Pelvic Inflammation Model Rats and Its Influence on TGF-β1 and MMP-2
摘要
Abstract
Objective:To explore the anti-inflammatory and anti-adhesion mechanisms of Gao's pelvic inflammation formula No.1 in chronic pelvic inflammation model rats and its effect on the cytokines TGF-β1 and MMP-2.Methods:A total of 75 female SD rats(12 weeks old)were divided into a blank group(10 rats)and a model group(65 rats).The pelvic inflammation model was established using a 25%phenol gel chemical burn combined with mechanical injury.Ten days after modeling,five rats from the model group were randomly selected for dissection to observe the modeling condition.The remaining 60 rats were randomly assigned into the model group,the Puling group,the placenta injection group,and the high,medium,and low dose Chinese medicine groups(each with 10 rats).The model group was given physiological saline 2 mL/(100g∙d)by gavage;the Chinese medicine groups were given Gao's Pelvic Inflammation Formula No.1 at doses of 7,14,and 28 g/(kg∙d);the Puling group was given Puling Pelvic Inflammation Granules 3 g/(kg∙d)by gavage;the placenta injection group received human placenta tissue fluid 1 mL/(kg∙d)by intramuscular injection,continuously for 20 days.Light microscopy was used to observe the uterine tissue morphology,ELISA was used to detect serum levels of TGF-β1 and IL-10,and immunohistochemistry was used to detect the expression of TGF-β1 and MMP-2 proteins in uterine tissue.Results:Light microscopy showed that the uterine walls in the model group rats thickened,the endometrial tissue was congested and edematous,the tissue structure was disordered,the glands were deformed,and inflammatory cell infiltration was observed,indicating successful modeling.ELISA detection of serum TGF-β1 and IL-10 levels showed that compared to other groups,the model group had significantly reduced IL-10 and elevated TGF-β1 levels(P<0.05).Compared to the model group,the Chinese medicine groups(high,medium,and low doses),the placenta injection group,and the Puling group all had elevated IL-10 and decreased TGF-β1 levels(P<0.05).No significant differences were observed between the high and medium dose Chinese medicine groups,the blank group,and the placenta injection group(P>0.05).Immunohistochemistry showed that the model group had decreased MMP-2 and increased TGF-β1 expression levels(P<0.05).Compared to the model group,the Chinese medicine groups(high,medium,and low doses)showed increased MMP-2 expression and decreased TGF-β1 expression(P<0.05).There was no significant difference between the high and medium dose groups,the blank group,and the placenta injection group(P>0.05),but significant differences were found between the high and medium dose Chinese medicine groups and the Puling group(P<0.05).Conclusion:The chronic pelvic inflammation model in rats was successfully replicated using phenol gel chemical burn combined with mechanical injury.After treatment with Gao's Pelvic Inflammation Formula No.1,the uterine tissue was repaired,inflammation and fibrosis were alleviated,and adhesion was prevented.The mechanism of action involves Gao's Pelvic Inflammation Formula No.1 increasing the inflammatory and adhesion-related factors IL-10 and MMP-2,while reducing the levels of inflammatory and adhesion cytokine TGF-β1.关键词
高氏盆炎方一号方/慢性盆腔炎/转化生长因子-β1/基质金属蛋白酶-2/抗炎/抗粘连Key words
Gao's Pelvic Inflammation Formula No.1/Chronic pelvic inflammation/Transforming growth factor-β1/Matrix metalloproteinase-2/Anti-inflammatory/Anti-adhesion引用本文复制引用
侯金萌,郝艳红,徐文君,刘玉兰,高慧,张晴,龙森,吴松柏..高氏盆炎方一号方对慢性盆腔炎模型大鼠抗炎作用及其对TGF-β1、MMP-2的影响[J].中医药信息,2025,42(4):12-18,7.基金项目
国家中医药管理局全国名老中医药专家高慧传承工作室建设项目(2015048157) （2015048157）
河北省中医药重点学科(中医妇科学)建设项目(202200505334) （中医妇科学）
河北省中医药管理局中医药类科研计划项目(2017082) （2017082）
河北省中医药管理局中医药类科研计划项目(2018164) （2018164）
