中医药信息2025，Vol.42Issue(4)：24-28,5.DOI:10.19656/j.cnki.1002-2406.20250404
基于Wnt/β-catenin信号通路探讨绿原酸对幽门螺杆菌相关性胃炎模型大鼠的抑菌作用及胃黏膜保护效应
Bacteriostasis and Gastric Mucosal Protective Effects of Chlorogenic Acid in Helicobacter pylori-Associated Gastritis Rats Based on the Wnt/β-Catenin Signaling Pathway
摘要
Abstract
Objective:To investigate the therapeutic effects of chlorogenic acid on Helicobacter pylori(H.pylori)-associated gastritis in rats,based on the Wnt/β-catenin signaling pathway,and explore its mechanism of action.Methods:A rat model of H.pylori-induced gastritis was established using a combination of H.pylori and the chemical carcinogen MNNG.After successful modeling,the rats were divided into four groups:the model group,the positive control group,the high-dose chlorogenic acid group(100 mg/kg),and the low-dose chlorogenic acid group(50 mg/kg),with normal controls.Each group consisted of 10 rats,and the treatment lasted for 4 weeks.The degree of H.pylori colonization in the gastric tissue of each group was evaluated using the rapid urease test.Histopathological examination of the gastric mucosa was conducted by optical microscopy.The levels of inflammatory cytokines IL-8,TNF-α in serum were determined by enzyme-linked immunosorbent assay(ELISA).The expression of Wnt2 and β-catenin genes in the gastric mucosa was detected by RT-qPCR.Results:Compared to the model group,the high and low-dose chlorogenic acid groups showed significant improvements in gastric mucosal congestion,edema,glandular arrangement,and inflammatory cell infiltration.The degree of H.pylori colonization was significantly reduced in both the high and low-dose chlorogenic acid groups,with eradication rates of 50%and 33%,respectively(P<0.01).Serum levels of IL-8 and TNF-α in the high and low-dose chlorogenic acid groups were significantly lower(P<0.01),and the relative gene expression of Wnt2 and β-catenin in the gastric mucosa was significantly downregulated(P<0.01).The serum levels of IL-8 and TNF-α in the chlorogenic acid groups were higher than those in the positive control group,while the relative gene expression of Wnt2 in the gastric mucosa was significantly lower than in the positive control group(P<0.01).Conclusion:Chlorogenic acid has therapeutic effects on H.pylori-associated gastritis in rats.It can improve H.pylori colonization and protect the gastric mucosa.The mechanism may involve inhibiting the abnormal activation of the Wnt/β-catenin pathway and downregulating the expression of IL-8 and TNF-α.关键词
绿原酸/幽门螺杆菌相关性胃炎/白细胞介素8/肿瘤坏死因子α/Wnt/β联蛋白信号转导通路Key words
Chlorogenic acid/Helicobacter pylori-associated gastritis/Interleukin 8/Tumor necrosis factor α/Wnt/β-catenin signaling pathway引用本文复制引用
吴迪,丁姣姣,王隆轩,张国斌..基于Wnt/β-catenin信号通路探讨绿原酸对幽门螺杆菌相关性胃炎模型大鼠的抑菌作用及胃黏膜保护效应[J].中医药信息,2025,42(4):24-28,5.基金项目
河南省高校科技创新人才支持计划项目(15HASTIT041) （15HASTIT041）
