安徽农业科学2025，Vol.53Issue(8)：1-6,6.DOI:10.3969/j.issn.0517-6611.2025.08.001
利用新型含烟叶培养基鉴定津巴布韦烟草叶面优势微生物群落
Identification of Dominant Microbial Communities on the Foliage of Zimbabwean Tobacco Using a Novel Tobacco-containing Medi-um
摘要
Abstract
In order to better identify the dominant microorganisms on the leaves of Zimbabwean tobacco,tobacco components at concentrations(volume fractions)of 0%,2.5%,5.0%and 10.0%were added to simulate the microbial growth environment on the surface of tobacco leaves,to screen the concentration of tobacco leaves suitable for microbial growth,to explore suitable tobacco culture medium preparation conditions,to analyze the differences in the composition of the microbial communities of tobacco leaves at different concentrations,to isolate and identify spe-cific microorganisms.The results showed that more specific microbial communities could be isolated when the tobacco leaf culture medium was adjusted to pH 7.0 at concentrations of 2.5%and 5.0%after sterilization.Metagenomic sequencing technology was used to determine the com-munity distribution of microorganisms on the surface of tobacco leaves in Zimbabwe.It was found that Bacillus had a high relative abundance in the culture medium at concentrations of 0%,2.5%and 5.0%,and after the addition of tobacco to the culture medium,Terribacillus became the dominant species.The genera with higher relative abundance on tobacco culture medium at concentrations of 2.5%and 5.0%included Pisciba-cillus,Amphibacillus,Margalitia,etc.Through isolation and sequencing,15 kinds of dominant microorganisms on the surface of tobacco leaves were identified,such as Bacillus species of the genus Bacillus,Bacillus veleis and Bacillus subtilis.关键词
烟草培养基/津巴布韦烟叶/宏基因组/优势微生物群落Key words
Tobacco-containing medium/Zimbabwean tobacco/Metagenomics/Dominant microbial community分类
轻工业引用本文复制引用
邱燕玲,周瑢,朱鑫权,徐艺雯,陶红,潘晓薇,欧阳璐斯..利用新型含烟叶培养基鉴定津巴布韦烟草叶面优势微生物群落[J].安徽农业科学,2025,53(8):1-6,6.基金项目
广东中烟工业有限责任公司和中山大学横向科研项目(粤烟工2022科字第29号). （粤烟工2022科字第29号）
