安徽农业科学2025，Vol.53Issue(8)：70-75,6.DOI:10.3969/j.issn.0517-6611.2025.08.015
鸽源大肠杆菌的分离鉴定·耐药性及致病性分析
Isolation,Identification,Drug Resistance,and Pathogenicity Analysis of Escherichia coli from Pigeons
摘要
Abstract
[Objective]To provide reference for the prevention and control of Escherichia coli disease and clinical medication in pigeon breed-ing.[Method]The isolated strains were analyzed through bacterial isolation and identification,drug sensitivity testing,animal pathogenicity tes-ting,and detection of resistance genes and virulence genes.[Result]The results showed that one strain of Escherichia coli was isolated from the liver tissue of deceased pigeons,one strain from the anal swab of diarrheal pigeons,and one strain from the dead embryo pigeon egg,named E.coli-SW,E.coli-FX,E.coli-SP.Three strains of Escherichia coli were sensitive to cefotaxime,ceftriaxone,and amikacin,and resistant to 15 other drugs.The resistance genes of TEM,aacC2,aacC4,qnrA,sul1,sul2,floR,and tetA could be detected.Three strains of Escherichia coli could kill mice,the virulence genes fyuA and irp2 were detected in E.coli-SW and E.coli-FX,while no virulence genes were detected in E.coli-SP.[Con-clusion]Three isolated strains of Escherichia coli have multiple drug resistance and certain pathogenicity,providing reference for the prevention and research of Escherichia coli disease of pigeons in the local area.关键词
鸽/大肠杆菌/分离鉴定/耐药性/致病性Key words
Pigeon/Escherichia coli/Isolation and identification/Drug resistance/Pathogenicity分类
畜牧业引用本文复制引用
黄运福,刘伟,彭婷,颜国庆,唐作顺,兰天艳,曾雪颜,银慧慧,赵武,姜源明,俸祥仁..鸽源大肠杆菌的分离鉴定·耐药性及致病性分析[J].安徽农业科学,2025,53(8):70-75,6.基金项目
南宁市科学研究与技术开发计划(20232058,20232050) （20232058,20232050）
2023年广西农业职业技术大学科研项目(XKJ2332) （XKJ2332）
广西基本科研业务费项目(桂科专项24-15) （桂科专项24-15）
广西兽医生物技术重点实验室自主研究课题(24-035-32-A-05). （24-035-32-A-05）
