北京中医药大学学报2025，Vol.48Issue(4)：459-470,12.DOI:10.3969/j.issn.1006-2157.2025.04.003
基于PI3K/Akt/NF-κB通路研究百秋李醇对肺热证小鼠的改善作用
Ameliorative effect of patchouli alcohol on mice with lung-heat syndrome based on PI3K/Akt/NF-κB pathway
摘要
Abstract
Objective To investigate the therapeutic effect of patchouli alcohol on mice with lung-heat syndrome based on the phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)/nuclear factor-kappa B(NF-κB)signaling pathway.Methods First,network pharmacology was used to predict the potential targets of patchouli alcohol in the treatment of lung-heat syndrome,and a"component-disease-key target"network was constructed for pathway analysis.Then,40 BALB/c mice were assigned to the normal,lung-heat model,honeysuckle,and low-dose and high-dose patchouli alcohol groups.All groups,except the blank group,were intranasally infected with 50 μL(103 TCID50)of influenza virus solution.After two hours of infection,mice were treated once a day for seven consecutive days.The therapeutic mechanism of patchouli alcohol was explored by measuring pulmonary inflammatory factors,the PI3K/Akt/NF-κB pathway,hypothalamic fever markers(PGE2,cAMP,cGMP levels),rectal temperature,and tissue energy metabolism.Results Network pharmacology identified 135 target genes related to patchouli alcohol and lung-heat syndrome,with the key targets being STAT3,H1F1A,and NF-κB1.In animal experiments,patchouli alcohol significantly alleviated influenza virus-induced lung inflammatory damage in mice with lung-heat syndrome,inhibited the expression of TNF-α and IL-6 in lung tissues(P<0.01),and suppressed the activation of the PI3K/Akt/NF-κB pathway.It also reduced hypothalamic levels of PGE2 and cAMP(P<0.01),suppressed the increase in rectal temperature,significantly decreased liver glycogen and pyruvate levels(P<0.01),and increased the activities of SDH,LDH,and Na+-K+-ATPase in the liver(P<0.01).Conclusion Patchouli alcohol improves the symptoms of lung-heat syndrome in mice by inhibiting the activation of the PI3K/Akt/NF-κB pathway,reducing proinflammatory cytokines and inflammatory damage,and regulating hypothalamic fever markers and energy metabolism.关键词
百秋李醇/肺热证/磷脂酰肌醇 3 激酶/Akt/核转录因子-κB 通路/能量代谢/芳香中药/小鼠Key words
patchouli alcohol/lung-heat syndrome/phosphoinositide 3-kinase/protein kinase B/nuclear factor-kappa B pathway/energy metabolism/aromatic Chinese herbs/mice分类
中医学引用本文复制引用
李林泽,张建军,王林元,李毅,乔灏祎,焦家康,张奇,伍小芳,赵星宇,赵尹铭,王淳..基于PI3K/Akt/NF-κB通路研究百秋李醇对肺热证小鼠的改善作用[J].北京中医药大学学报,2025,48(4):459-470,12.基金项目
国家自然科学基金项目(No.82141217) National Natural Science Foundation of China(No.82141217) （No.82141217）
