草业学报2025，Vol.34Issue(4)：104-123,20.DOI:10.11686/cyxb2024185
NaCl胁迫下鹰嘴紫云英根系基因差异表达及相关通路分析
Differentially expressed genes and related pathways in root systems of Astragalus cicer under NaCl stress
摘要
Abstract
Soil salinization seriously affects the growth and yield of plants,and limits the development of agricultural and ecological economies.Astragalus cicer is a versatile ecological grass species,an efficient green manure crop,and a superior protein forage.Exploring the molecular mechanism of its adaptability to NaCl stress is of great significance for enhancing its salt tolerance and advancing its cultivation in saline soil.To investigate its responses to NaCl stress,transcriptomic analyses were performed on the roots of A.cicer at different time points(0,12,48,and 72 hours)during an NaCl treatment.The results showed that,compared with 0 hour,after 12,48,and 72 hours of NaCl stress,there were,respectively,46051,45653,and 42869 differentially expressed genes(DEGs)in the roots of A.cicer,including 8027,10053,and 11042 up-regulated DEGs and 38024,35600,and 31827 down-regulated DEGs,respectively,at the three time points.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that the pathways responding to NaCl stress in the roots of A.cicer were oxidoreductase activity,flavonoid biosynthesis,and phenylpropanoid biosynthesis.A trend analysis showed that the DEGs in phenylpropanoid biosynthesis and flavonoid biosynthesis pathway were still up-regulated or had returned to their baseline levels at 48 hours of NaCl treatment.Genes encoding C2H2,C3H,NAC,MYB,WRKY,and bZIP transcription factors were expressed at different time points during the NaCl treatment,suggesting that these transcription factors are closely related to salt tolerance in A.cicer.The results of this study provide basic data for further exploration of the mechanism of salt tolerance of A.cicer,and also provide theoretical support for breeding to improve salt tolerance.关键词
鹰嘴紫云英/根系/转录组/NaCl胁迫/差异表达基因/代谢通路Key words
Astragalus cicer/roots/transcriptome/NaCl stress/differentially expressed genes/metabolic pathway引用本文复制引用
马婷,陈奋奇,王勇,哈雪,李亚君,马晖玲..NaCl胁迫下鹰嘴紫云英根系基因差异表达及相关通路分析[J].草业学报,2025,34(4):104-123,20.基金项目
苜蓿育种项目(2022ZD0401102)资助. （2022ZD0401102）
