草业学报2025，Vol.34Issue(4)：124-136,13.DOI:10.11686/cyxb2024206
紫花苜蓿LACS基因家族成员鉴定及表达分析
Identification and expression analysis of LACS gene family members in Medicago sativa
摘要
Abstract
Members of the long-chain acyl-coenzyme A(CoA)synthetase(LACS)family,in the acyl-activating enzyme superfamily,play important roles in fatty acid anabolic metabolism.In this study,based on genomic data of Medicago sativa(alfalfa),LACS gene family members were identified by bioinformatics methods,a phylogenetic tree was constructed,and the physicochemical properties of the putative proteins were determined.The chromosome localization,conserved motifs and gene structure,cis-acting elements,and tissue-specific expression patterns of the LACS genes were analyzed,and a protein-protein interaction(PPI)network was constructed.The transcript profiles of LACS genes under biotic stress were analyzed by qRT-PCR.The results showed that 10 MsLACS family members were present in the alfalfa genome,and were located on five chromosomes.In the phylogenetic tree,the 10 MsLACS were grouped into five branches.Motif3 constituted the conserved AMP-binding domain,and the alfalfa MsLACS genes contained 10-12 motifs.There were differences in gene structure among the 10 MsLACS genes,with the number of exons ranging from 11 to 22,and the number of introns ranging from 0 to 3.MsLACS1-1 and MsLACS3 had no introns.The promoter region of MsLACS contained light response elements,hormone response elements,and abiotic stress response elements.The transcript profiles of MsLACS genes differed among different tissues and showed obvious tissue specificity.Analyses of gene expression by qRT-PCR revealed higher transcript levels ofMsLACS genes under drought stress and salt stress than under cold stress.Under cold stress,the transcript levels ofMsLACS genes in alfalfa initially increased and then decreased,and were higher in the leaves than in the roots.Under drought and salt stress,MsLACS genes were highly expressed in leaves,with peak transcript levels at 6 h.The PPI network analysis showed that the 10 proteins encoded by MsLACS genes in alfalfa interacted with each other,with 18 lines of interaction among the proteins.These results provide a basis for further research on LACS genes in alfalfa and their applications in breeding for stress resistance.关键词
紫花苜蓿/长链脂酰辅酶A合成酶(LACS)/基因家族/非生物胁迫/表达Key words
Medicago sativa/long-chain acyl-coenzyme A(CoA)synthetase(LACS)/gene family/abiotic stress/expression引用本文复制引用
罗天蓉,马健芝,杜明阳,多杰措,熊辉岩,段瑞君..紫花苜蓿LACS基因家族成员鉴定及表达分析[J].草业学报,2025,34(4):124-136,13.基金项目
青海省重点研发与转化计划(2022-NK-135)和青海省高原紫花苜蓿品种适应性评价与示范资助. （2022-NK-135）
