草业学报2025，Vol.34Issue(4)：137-149,13.DOI:10.11686/cyxb2024256
基于重测序紫花苜蓿高蛋白、高产关联InDel分子标记开发
Development of high-protein and high-yield associated InDel molecular markers based on re-sequencing in alfalfa
摘要
Abstract
With the rapid development of high-throughput sequencing technology,the sequencing cost has been greatly reduced,and the sequencing speed and data quality have been significantly improved.These developments greatly promote the in-depth study of the alfalfa(Medicago sativa)genome,and thus provide important basic information for the development of molecular markers for the association of different traits in alfalfa.Insertion/deletion(InDel)polymorphic markers have attracted great attention because of their wide distribution,high density,genetic stability,and high reproducibility,but research about them and their application in alfalfa are still very limited.In this study,based on genome resequencing data of 80 alfalfa germplasm lines sourced from China and abroad,we explored the InDel locus by comparing with the reference genome sequence of alfalfa"Zhongmu No.1",and identified InDel markers related to high-protein and high-yield traits.It was found that of 40 markers identified on a genome-wide scale,29 polymorphic markers were successfully categorised with a polymorphism ratio of 72.5％after validation by polymerase chain reaction(PCR).Notably,markers designated InDel_25 and InDel_39 are particularly useful for the identification of high-yield and high-quality alfalfa germplasm lines,respectively.It is anticipated that the InDel markers identified in this research will be applied in traditional breeding programs,thereby accelerating the development of new alfalfa varieties with enhanced yield and quality.关键词
紫花苜蓿/InDel分子标记/产量/品质Key words
alfalfa/InDel molecular markers/yield/quality引用本文复制引用
冯雅琪,陈嘉慧,张静妮,隋超,陈基伟,刘志鹏,周强,刘文献..基于重测序紫花苜蓿高蛋白、高产关联InDel分子标记开发[J].草业学报,2025,34(4):137-149,13.基金项目
农业生物育种国家科技重大专项,国家自然科学基金项目(32441032 （）
32271748)和财政部和农业农村部:国家现代农业产业技术体系资助. （）
