草业学报2025，Vol.34Issue(4)：175-188,14.DOI:10.11686/cyxb2024194
三株丹参根内生细菌对宿主生长及药用品质的影响
Effects of three endophytic bacteria in the roots of Salvia miltiorrhiza on host growth and medicinal quality
摘要
Abstract
A pot experiment was conducted to study the effects of three endophytic bacteria on the growth,physiology,and medicinal quality of Salvia miltiorrhiza.The ultimate aim of this research was to identify bacteria suitable for the development of microbial fertilizers for S.miltiorrhiza.Three endophytic bacteria(Mesorhizobium amorphae B546,Bacillus thuringiensis NB49,and B.thuringiensis Bt12)isolated from the roots of S.miltiorrhiza were applied to seedlings via root irrigation.The growth and physiological indicators were measured at different times(7,14,28,42,56 d)after treatment,and the contents of eight active ingredients in the roots were determined at harvest.It was found that all three endophytic bacteria significantly promoted the accumulation of S.miltiorrhiza biomass.After 56 days of growth,the diameter of the main root and the dry weight of the above ground and underground parts were significantly higher(by 1.21-2.49 times)in all the treatments than in the uninoculated control(CK)(P＜0.05).The endophytic bacterial treatments had a significant impact on the physiology of S.miltiorrhiza,with B.thuringiensis Bt12 having the best promoting effect.After 42 days of growth,the contents of soluble sugars,soluble proteins,total chlorophyll,zein,6-benzylaminopurine,and auxin were 1.36-to 2.18-times higher in the treatments than in CK.The three endophytic bacteria strains exerted distinct effects on the accumulation of active components in S.miltiorrhiza.Notably,B.thuringiensis Bt12 significantly enhanced the accumulation of total tanshinone and total salvianolic acid to levels 2.10-and 1.37-times that in CK,respectively.M.amorphae B546 and B.thuringiensis NB49 promoted the accumulation of total tanshinone,to levels 1.08-and 1.34-times that in CK,respectively,but inhibited the accumulation of phenolic acids,with the total phenolic acid content being 92.21％and 69.29％of that in CK,respectively.In summary,the three strains of endophytic bacteria significantly affected the growth,physiological metabolism,and accumulation of active ingredients in S.miltiorrhiza.Among the tested endophytic bacteria,B.thuringiensis Bt12 had the strongest promoting effect.Thus,it has potential applications in the development of specialized microbial fertilizers for S.miltiorrhiza.关键词
丹参/内生菌/微生物肥料/促生长/药用品质Key words
Salvia miltiorrhiza/endophytic bacteria/microbial fertilizers/promote growth/medicinal quality引用本文复制引用
马召,李晓帆,孙莉琼,黄智,许垒,鲁霆,唐晓清,王康才..三株丹参根内生细菌对宿主生长及药用品质的影响[J].草业学报,2025,34(4):175-188,14.基金项目
江苏省农业科技自主创新项目[CX(22)3174],中央高校基本科研业务费(KYZZ2022002),南京农业大学中药全产业链(KYCXJC2023003)和企业合作项目(20231020)资助. （22）
