草业学报2025，Vol.34Issue(4)：201-211,11.DOI:10.11686/cyxb2024157
纳米铁与铜对苜蓿壳二孢及其引致春季黑茎病的抑制与防治作用
Inhibition and control effect of nano-iron and copper on Ascochyta medicaginicola and spring black stem disease
摘要
Abstract
Alfalfa(Medicago sativa),the most extensively cultivated leguminous forage in China and globally,is susceptible to spring black stem disease caused by Ascochyta medicaginicola,one of the five most devastating diseases of alfalfa in China.Among various nanoparticles tested for their antifungal properties against A.medicaginicola,copper nanoparticles(Cu NPs)demonstrated the most pronounced inhibitory effect,with an inhibition rate of 74.1％at a concentration of 400 mg·L-1.The application of Cu NPs also significantly mitigated the impact of spring black stem disease on alfalfa,reducing the relative lesion area to 5.95％when applied prior to inoculation with a spore suspension,and to 8.75％when applied after inoculation with a spore suspension.When alfalfa leaves were pre-treated with a Cu NPs suspension,the relative conductivity was only 18.11％,contrasting sharply with the 82.27％relative conductivity of alfalfa leaves inoculated with a spore suspension only.The enzymatic responses in Cu NPs-treated alfalfa were notable,with a significant decrease in the activities of superoxide dismutase and phenylalanine ammonia-lyase compared with the spore-inoculated control group.Conversely,the activity of polyphenol oxidase was increased by 42.6％,while catalase activity decreased by 45.0％in the first Cu NPs inoculation treatment.Ultrastructural analyses revealed that Cu NPs punctured and caused visible damage to the cell membrane of A.medicaginicola within alfalfa leaves.In contrast,the chloroplast thylakoids in the treated leaves remained clearly layered,indicating that alfalfa cell membrane integrity remained intact.These findings offer valuable insights into the potential use of Cu NPs as a control measure for alfalfa spring black stem disease.关键词
紫花苜蓿/纳米铜/苜蓿壳二孢/抑制/防治Key words
Medicago sativa/Cu NPs/Ascochyta medicaginicola/inhibition/control effect引用本文复制引用
董拓轩,陈训锋,梅大海,郭永莎,魏旭红,宋秋艳..纳米铁与铜对苜蓿壳二孢及其引致春季黑茎病的抑制与防治作用[J].草业学报,2025,34(4):201-211,11.基金项目
"十四五"重点研发计划(2023YFD1301202,2022YFD1300802),甘肃省自然科学基金(23JRRA1076),国家自然科学基金(31901388),国家现代农业产业技术体系(CARS-34)和兰州大学中央高校基本科研业务费(lzujbky-2024-ou15)专项资金资助. （2023YFD1301202,2022YFD1300802）
