茶叶学报2025，Vol.66Issue(1)：55-64,10.DOI:10.20045/j.cnki.issn.2096-0220.2025.01.007
基于微卫星分子标记的福建省灰茶尺蠖遗传分化分析
Microsatellite Markers-based Genetic Differentiation of Ectropis grisescens Populations in Fujian Province
摘要
Abstract
[Objective]Ectropis grisescens Warren,a common leaf-eating pest in tea plantations,was widely distributed in various tea producing cities in Fujian Province and caused certain economic losses.To develop and design primers for microsatellite loci of grey tea inchworm,and to study the genetic differences of different geographical populations in Fujian Province,in order to understand their population occurrence rules and provide references for the development of sustainable prevention and control technologies.[Method]MISA was used to search the genome and transcriptome sequences of E.grisescens Warren for loci distribution.Primer 3 and TBtools were employed to design the primers for the simulated amplification.The three-primer fluorescence labeling method was applied to obtain the pairs of polymorphic microsatellite loci for differentiating the specimens of 4 population groups collected in the province.[Result]The repeat types of microsatellite loci in E.grisescens were numerous,and the repeat motifs showed apparent AT bias.The 4 population groups significantly deviated from the Hardy-Weinberg equilibrium on part of the 5 identified pairs of polymorphic loci.[Conclusion]The genetic deviation in the E.grisescens population at Quanzhou was the greatest and that at Ningde significant among the 4 groups.There was no significant difference between those at Sanming and Nanping.关键词
灰茶尺蛾/微卫星分子标记/遗传多样性/种群分化/地理种群Key words
Ectropis grisescens/microsatellite markers/genetic diversity/population differentiation/geographical population groups分类
农业科学引用本文复制引用
陈钰洁,史凡,江晨楠,高晓寒,林伊缤,李文韬,陈李林..基于微卫星分子标记的福建省灰茶尺蠖遗传分化分析[J].茶叶学报,2025,66(1):55-64,10.基金项目
福建省自然科学基金(引导性项目,2024N0053 （）
对外合作项目,2022I0009) （）
南平市科技特派员专项经费(N2023T011) （N2023T011）
福建农林大学大学生创新训练项目(202410389014,FAFUXMPC20240507002-00036). （202410389014,FAFUXMPC20240507002-00036）
