福建农林大学学报（自然科学版）2025，Vol.54Issue(3)：299-308,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202407032
木薯MebZIP11基因克隆与原核表达分析
Cloning and prokaryotic expression analysis of MebZIP11 gene in cassava(Manihot esculenta)
摘要
Abstract
[Objective]This study aimed to investigate the function of Manihot esculenta basic leucine zipper11(MebZIP11)gene,and provide insights into the role of A-type bZIP transcription factor in M.esculenta(cassava)in response to abiotic stress.[Meth-od]The MebZIP11 gene from cassava was cloned using RT-PCR technology,followed by bioinformatics analysis.The expression pat-tern of MebZIP11 was analyzed in various cassava tissues and at different stages of root tuber development via qRT-PCR,in combi-nation with the evaluation on the gene′s response to various stress conditions.[Result]The MebZIP11 gene is located on chromosome 5 of cassava,with a coding region of 1 221 bp in length,exhibiting typical bZIP structural features.Sequence alignment showed that MebZIP11 shared 80.00％and 85.41％homology with the A-type bZIP sequences of Jatropha curcas and Hevea brasiliensis,respec-tively,confirming its belonging to the A-type bZIP subfamily.Subcellular localization assays demonstrated that the MebZIP11 protein was localized in the nucleus,and the MebZIP11 was most expressed in the storage roots by expression analysis.Under different treat-ment conditions,the expression of MebZIP11 was significantly induced by mannitol,NaCl and abscisic acid,while was suppressed by H2O2 and cold treatment.Lastly,the MebZIP11 protein was successfully expressed and purified in prokaryotic system.[Conclu-sion]The MebZIP11 gene in cassava is identified as a member of A-type bZIP family.It exhibits distinct inducible responses under various stress and hormone treatments.关键词
木薯/非生物胁迫/碱性亮氨酸拉链/逆境响应/原核表达Key words
cassava/abiotic stress/bZIP/stress response/prokaryotic expression分类
农业科学引用本文复制引用
雷鑫芳,潘子墨,林素妍,罗佳科,吴春来,曾坚,胡伟..木薯MebZIP11基因克隆与原核表达分析[J].福建农林大学学报（自然科学版）,2025,54(3):299-308,10.基金项目
广东省普通高校重点领域专项(2024ZDZX4018,2022ZDZX4047) （2024ZDZX4018,2022ZDZX4047）
广东省基础与应用基础研究基金项目(2025A1515010531,2023A1515010336) （2025A1515010531,2023A1515010336）
韶关学院重点项目(SZ2022KJ05) （SZ2022KJ05）
大学生创新创业训练计划项目(Sycxcy2024176). （Sycxcy2024176）
