福建农林大学学报（自然科学版）2025，Vol.54Issue(3)：325-334,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202407027
TPPP2基因在保山猪睾丸中的表达、转录调控及潜在的生物学作用机制
Expression,transcriptional regulation and potential biological mechanism of TPPP2 gene in the testis of Baoshan swine
摘要
Abstract
[Objective]This study aimed to investigate the basic biological characteristics of tubulin polymerization promoting protein family member 2(TPPP2)gene in the testis of Baoshan swine,as well as its expression levels in other tissues,so as to provide a basis for elucidating its mechanism in Baoshan swine.[Method]The expression of TPPP2 gene in the testes of Baoshan swine was analyzed at different months through whole-transcriptome sequencing.After constructing a competitive endogenous RNA(ceRNA)regulatory network between TPPP2 mRNA,miRNA,and lncRNA,the amino acid homology and protein interaction network of TP-PP2 were analyzed.Moreover,GO and KEGG enrichment analyses were performed on the interacting proteins of TPPP2,and their expression correlation with TPPP2 were analyzed through transcriptome sequencing data.[Result]The TPPP2 gene,located on chromosome 7,had a 516 bp coding sequence(CDS)and consisted of 4 exons and 3 introns.Functional analysis revealed that TP-PP2 comprised 171 amino acids,exhibited hydrophobicity,and contained phosphorylation sites.Phylogenetic homology and adjacent gene analyses indicated the clustering of TPPP2 in Baoshan swine with multiple species,which was in accordance with taxonomic standards;with sequence similarity exceeding 80％,it was considered of high conserved neighboring genes.Protein interaction anal-ysis revealed close interaction between TPPP2 and protein OAZ3 and NME8,and these interacting proteins were primarily enriched in pathways involved in T-cell receptor signaling,sphingolipid signaling and mRNA surveillance by KEGG enrichment analysis,and related to sperm flagellum motility,sperm DNA condensation,sperm motility and sperm nuclear differentiation by GO enrichment a-nalysis.Furthermore,TPPP2 was targeted and regulated by 3 miRNA(ssc-miR-150,ssc-miR-205 and ssc-miR-222).qPCR analy-sis revealed differential expression of TPPP2 across 15 tissues,with the highest expression in testis.Notably,its expression in testic-ular tissue progressively increased with age in Baoshan swine.[Conclusion]This study comprehensively characterized the molecular features,protein structure,and transcriptional regulatory network of the TPPP2 gene in Baoshan swine teste,highlighting its notably high expression in testis.These results demonstrated that TPPP2 gene plays an essential role in porcine spermatogenesis.关键词
微管蛋白聚合促进蛋白家族成员 2/猪/分子结构/蛋白特征/转录调控Key words
tubulin polymerization promoting protein family member 2/swine/molecular structure/protein characteristics/tran-scriptional regulation分类
畜牧业引用本文复制引用
翟兰,席学敏,常勇诚,赵加鼎,富国文,徐世雄,龚绍荣,赵桂英,霍金龙..TPPP2基因在保山猪睾丸中的表达、转录调控及潜在的生物学作用机制[J].福建农林大学学报（自然科学版）,2025,54(3):325-334,10.基金项目
云南省科技厅重大科技专项(202302AE090016) （202302AE090016）
云南省施甸县保山猪产业科技特派团项目(202304BI090010) （202304BI090010）
云南省财政厅科教文化专项(A3032022211) （A3032022211）
云南省科技厅重点研发项目(2018BB003). （2018BB003）
