福建农林大学学报（自然科学版）2025，Vol.54Issue(3)：335-343,9.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202406033
芦荟大黄素、姜黄素对IncX4型耐药质粒pSD11接合转移的影响
Effect of aloe-emodin and curcumin on conjugation transfer of IncX4 type resistance plasmid pSD11
摘要
Abstract
[Objective]The effects and mechanism of aloe-emodin and curcumin on conjugation transfer of IncX4 type resistance plasmid pSD11 were studied to provide reference for the application of traditional Chinese medicine monomers on inhibiting plasmid conjugation transfer and thus lowering bacterial drug resistance.[Method]The conjugation transfer efficiency of plasmid pSD11 was determined by membrane conjugation transfer and plate counting under aloe-emodin and curcumin at subinhibitory concentration.And their inhibitory mechanism on plasmid conjugation transfer were studied by analyzing the expression of conjugation transfer-relat-ed genes through quantitative real-time PCR(qRT-PCR)and biofilm formation ability of resistant strain.[Result]Aloe-emodin and curcumin at the subinhibitory concentration could retard the conjugation transfer of plasmid pSD11.At the effective concentration of inhibiting conjugation transfer,aloe-emodin and curcumin can both up-regulate the expression of key genes in the plasmid type Ⅳsecretion system.Aloe-emodin can inhibit the expression of chromosomal conjugation transfer-related gene h-ns,cpxR and pilT,while curcumin exerted multiple effects on their expressions at different concentrations.Furthermore,aloe-emodin can inhibit the biofilm formation of drug-resistance bacteria MG1655/pSD11,while curcumin suppressed biofilm formation at high concentration and facilitated the process at low concentration.[Conclusion]The inhibition of aloe-emodin and curcumin on the conjugation transfer of plasmid pSD11 was not the consequence of regulated expression of key genes involved in type Ⅳ secretion system.In general,the inhibitory mechanism of aloe-emodin includes the down-regulation of pilT gene and biofilm inhibition of drug-resistant bacteria,while curcumin at different concentrations acted through multiple effects on cpxR and pilT expressions and biofilm formation.关键词
中药单体/IncX4 型质粒/接合转移/细菌生物膜/细菌耐药性Key words
traditional Chinese medicine monomer/IncX4 plasmid/conjugation transfer/bacterial biofilm/bacterial drug resist-ance分类
畜牧业引用本文复制引用
沈雪,甘德浩,陈璐,郑婷婷,范克伟,邓辉..芦荟大黄素、姜黄素对IncX4型耐药质粒pSD11接合转移的影响[J].福建农林大学学报（自然科学版）,2025,54(3):335-343,9.基金项目
福建省家畜传染病防治与生物技术重点实验室开放基金课题(2021ZDKF02). （2021ZDKF02）
