高等学校化学学报2025，Vol.46Issue(5)：1-10,10.DOI:10.7503/cjcu20250023
外泌体处理温度对其蛋白质组学分析的影响
Effect of Exosome Treatment Temperature on Proteomic Analysis of Exosomes
摘要
Abstract
Exosome proteomic analysis has important value in screening biomarkers for major diseases,discovering drug targets,and studying functional mechanisms.Exosome and protein are widely recognized as research objects that are highly susceptible to temperature changes.However,previous reports have often attributed the differences in exosome proteomic analysis results based on different enrichment strategies directly to the differences in enrichment selectivity and efficiency of different enrichment methods.There has not been sufficient research and discussion on the impact of temperature changes during the enrichment process of exosome on the results of exosome proteomic analysis.To investigate the effect of sample processing temperature on the results of exosome proteomics analysis,this study treated exosome samples at six temperature conditions of 4,25,37,45,60,and 90℃ for 1 h.Subsequently,the morphology characteristics,particle size distribution and concentration,changes in characteristic protein characterization content,and proteomic data of the samples were systematically analyzed.The results showed that there were no significant differences in morphology and particle size among exosomes treated at different temperatures,But as the temperature increases,the concentration of exosome particles significantly decreases.When the temperature exceeds 45℃,the characterization content of the exosome marker protein TSG101 significantly decreases,while conversely,the characterization content of the exosome characteristic membrane protein CD9 significantly increases.The results of quantitative proteomics analysis further indicate that the proteomic data of exosome samples treated at 4,25 and 37℃ have good comparability,while samples treated at 45℃ and above show significant differences in 48 proteins.In summary,when conducting exosomal proteomic analysis,the potential impact of temperature changes on measurement results should be fully considered to ensure the reliability,reproducibility,and comparability of the data.关键词
外泌体蛋白质组/温度/质谱Key words
Exosome proteomic/Temperature/Mass spectrometry分类
化学引用本文复制引用
吴梦鸽,楚占营,孟波,叶子弘,翟睿..外泌体处理温度对其蛋白质组学分析的影响[J].高等学校化学学报,2025,46(5):1-10,10.基金项目
中国计量科学研究院院基本科研业务费(批准号:AKYCX2211)和"万人计划"科技创新领军人才项目(批准号:WR2202)资助. Supported by the Fundamental Research Funds of the National Institute of Metrology(No.AKYCX2211)and the"Ten-Thousands Talents Program"Plan for Leading Talents of Science and Technology Innovation,China(No.WR2202). （批准号:AKYCX2211）
