高等学校化学学报2025，Vol.46Issue(5)：11-18,8.DOI:10.7503/cjcu20250008
聚胸腺嘧啶-铜纳米簇/适体-金纳米粒子荧光探针用于微囊藻毒素-LR传感分析
Fluorescent Probe for Detection of Microcystin-LR Based on Poly(thymidine)-Copper Nanoclusters and Aptamer-Gold Nanoparticles
摘要
Abstract
A fluorescent probe of poly(thymidine)-copper nanoclusters/aptamer-gold nanoparticles(poly(T)-CuNCs/aptamer-AuNPs)was constructed for highly sensitive sensing detection of microcystin-LR(MC-LR)using DNA template method.Three DNA nucleotides were designed,including MC-LR aptamer,and two poly(thymidine)ssDNA(poly(T)S1 and poly(T)S2).Using poly(T)S1 and poly(T)S2 as templates,poly(T)S1-CuNCs and poly(T)S2-CuNCs with pink fluorescence were synthesized by reducting Cu2+with ascorbic acid(AA).Aptamer labeled with thiol groups at two both ends were linked with AuNPs through Au—S bonds to form AuNPs-aptamer-AuNPs bioconjugates.AuNPs-aptamer-AuNPs hybridized with poly(T)-CuNCs to form dsDNA-CuNCs.Fluorescence resonance energy transfer(FRET)occurred between CuNCs and AuNPs in the dsDNA-CuNCs structure,leading to fluorescence quenching of dsDNA-CuNCs.In the presence of target MC-LR,MC-LR specifically bonded with aptamer in dsDNA-CuNCs,resulting in the dissociation of dsDNA structure.The poly(T)-CuNCs were released into the solution,restoring the system's fluorescence.An"off-on"type of fluorescent probe was constructed for the detection of MC-LR.The linear range for MC-LR detection is 1 ng/L—500 μg/L,with a detection limit of 0.3 ng/L(S/N=3).The proposed fluorescent aptamer probe possesses the advantages of simple preparation,high selectivity,and can be applied for quantitative detection and analysis of MC-LR in real water samples.关键词
微囊藻毒素-LR/聚胸腺嘧啶-铜纳米簇/适体-金纳米粒子/荧光探针Key words
Microcystin-LR/Poly(thymine)-copper nanoclusters/Aptamer-gold nanoparticles/Fluorescent probe分类
化学化工引用本文复制引用
蒙化,阿华英,罗平馨,张艳丽,高连训,王红斌,杨文荣,庞鹏飞..聚胸腺嘧啶-铜纳米簇/适体-金纳米粒子荧光探针用于微囊藻毒素-LR传感分析[J].高等学校化学学报,2025,46(5):11-18,8.基金项目
国家自然科学基金(批准号:21565031,21665027)、云南省科技厅基础研究计划项目(批准号:202001AT070012)和云南民族大学研究生科研创新基金(批准号:2024SKY135)资助. Supported by the National Natural Science Foundation of China(Nos.21565031,21665027),the Basic Research Project of Yunnan Provincial Science and Technology Department,China(No.202001AT070012)and the Graduate Research Innovation Project of Yunnan Minzu University,China(No.2024SKY135). （批准号:21565031,21665027）
