高等学校化学学报2025，Vol.46Issue(5)：19-28,10.DOI:10.7503/cjcu20240524
糖基印迹电化学传感器的制备及乳腺癌PD-L1阳性外泌体的快速检测
Development of a Glycosyl-imprinted Sensor and Rapid Detection of PD-L1 Positive Exosomes in Breast Cancer
摘要
Abstract
A glycosylated electrochemical sensor for rapid identification and detection of breast cancer PD-L1 positive exosomes was developed.First,glycosyl-imprinted polymers(GIP)were prepared by electropolymerization using glyco-Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Gal overexpressed by breast cancer positive exosome glycoprotein PD-L1 as template molecules and 3-aminophenylboronic acid as functional monomers.After elution and removal of template molecules,an imprinted membrane that can specifically recognize PD-L1 positive exosomes was obtained.Potassium ferricyanide was used as a probe to measure the DPV current value of the GIP electrode.Exosomes were cleaved with RIPA to deduct free protein interference,and the change in current value(ΔI)was recorded.ΔI decreased with the increase of the concentration of readsorbed PD-L1-positive exosomes,and was linearly positively related to the logarithmic value of the concentration.The detection range was 2.36×102-1.18×107 particles/mL,and the detection limit was 93 particles/mL.The method has been used to detect breast cancer PD-L1-positive exosomes in clinical samples,and its spiked recoveries were 93.82%—105.32%.The sensor could be used to screen breast cancer in clinical samples by the difference in glycosylation degree.关键词
外泌体/糖基印迹/电化学传感器/PD-L1/乳腺癌Key words
Exosome/Glycosyl-imprinting/Electrochemical sensor/PD-L1/Breast cancer分类
化学引用本文复制引用
罗奎,林珈希,李建平..糖基印迹电化学传感器的制备及乳腺癌PD-L1阳性外泌体的快速检测[J].高等学校化学学报,2025,46(5):19-28,10.基金项目
国家自然科学基金(批准号:22264010)资助. Supported by the National Natural Science Foundation of China(No.22264010). （批准号:22264010）
