高等学校化学学报2025，Vol.46Issue(5)：29-36,8.DOI:10.7503/cjcu20240517
荧光探针对植物组织乙烯释放的监测及在白熟期番茄中的应用
Monitoring of Ethylene Release from Plant Tissues by Fluorescent Probe and Application in White Ripe Tomato
摘要
Abstract
The quantification of ethylene can be achieved through the change of fluorescence signal,but there are few reports about the real-time monitoring of ethylene levels in plant tissues.Developing environmentally friendly and biocompatible chemical-based fluorescent probes is one of the ways to achieve plant tissue imaging.This article uses casein as a carrier to create a water-soluble fluorescent probe(C-E-P)by encapsulating a fluorescent probe(P3)that combines the first-generation Grubbs catalyst with 6-methoxy-2-naphthylene in a hydrophobic pocket.The synthesis of probe P3 was confirmed through various structural characterization methods.Performance tests demonstrated that P3 exhibited a strong linear relationship with ethylene,rapid reaction time,and high selectivity.The C-E-P,created by encapsulating P3 with casein,facilitated fluorescence imaging of ethylene release from apple,bitter water rose,and aloe tissues.This approach allowed for observing changes in ethylene release between different plant tissues over time.Additionally,it was employed to monitor ethylene variations in the tissues of white ripe tomatoes during ripening,revealing that the peak ethylene release from the seeds occurs before that from the pericarp.The C-E-P developed in this study can effectively capture ethylene release information in plant tissues,providing an effective tool for fruit ripening and plant health monitoring in agricultural production.关键词
植物组织荧光成像/乙烯/荧光探针/酪蛋白Key words
Plant tissue fluorescence imaging/Ethylene/Fluorescent probe/Casein分类
化学引用本文复制引用
杨倩,屠鹏,郝振芳,崔彦君,边红霞..荧光探针对植物组织乙烯释放的监测及在白熟期番茄中的应用[J].高等学校化学学报,2025,46(5):29-36,8.基金项目
甘肃省自然科学基金(批准号:22JR5RA866)资助. Supported by Gansu Provincial Natural Science Foundation,China(No.22JR5RA866). （批准号:22JR5RA866）
