寒旱农业科学2025，Vol.4Issue(4)：365-372,8.DOI:10.3969/j.issn.2097-2172.2025.04.014
生防菌株YM3-3 的鉴定和抑菌能力测定及发酵条件优化
Identification,Antagonistic Ability Evaluation,and Fermentation Optimization of Biocontrol Strain YM3-3
摘要
Abstract
To provide biocontrol strain resources for high-efficiency and safe microbial pesticides,the endophytic Bacillus subtilis YM3-3,a strain with ideal biocontrol potential,isolated from maize leaves was identified,and the fermentation conditions were optimized.This study provided technical parameters and a theoretical basis on control maize diseases as biocontrol agents.The inhibition effect of strain YM3-3 against 10 pathogenic fungi(including Fusarium verticillioides,Exserohilum turcicum,Mycosphaerella melonis,Valsa mali,Physalospora piricola,Sclerotinia sclerotiorum,Rhizoctonia solani,Pythiunaphanidermatum,Phytophthora capsici and Fusarium oxysporum f.sp.vesinfectum.)was measured by the dural culture method.The results showed that it had good antagonistic effect,and the inhibition rate against 10 pathogenic fungi was(53.19±3.78)%to(81.94±1.54)%,showing good biocontrol potential.According to morphological,16SrDNA,and gyrB sequence analysis,it was identified as Bacillus subtilis.In order to further optimize the fermentation conditions,the optimization experiment of culture conditions was carried out.The results showed that the optimal culture time,temperature,rotational speed,pH,and liquid volume were 3 d,27℃,150 r/min,7 and 90 mL,respectively.The optimal combination of fermentation medium was 15 g/L mannitol,2.5 g/L peptone,7.5 g/L potassium nitrate,and 5.0 g/L calcium carbonate.关键词
枯草芽孢杆菌/YM3-3/抑菌能力/发酵条件/正交试验/培养条件/优化组合Key words
Bacillus subtilis/YM3-3/Antagonistic ability/Fermentation condition/Orthogonal test/Culture condition/Optimization分类
生物科学引用本文复制引用
王春明,郭成,周天旺,洪流,徐生海,许永锋..生防菌株YM3-3 的鉴定和抑菌能力测定及发酵条件优化[J].寒旱农业科学,2025,4(4):365-372,8.基金项目
甘肃省科技计划-自然科学基金项目(24JRRA729) （24JRRA729）
甘肃省科技计划-科技特派员(团)专项(24CXNA048). （团）
