东南园艺2025，Vol.13Issue(1)：1-14,14.DOI:10.20023/j.cnki.2095-5774.2025.01.001
十字花科植物调控硫苷合成MYB转录因子的生物信息学分析
Bioinformatics analysis of MYB transcription factors regulating glucosinolate biosynthesis in Brassicaceae
摘要
Abstract
[Objective]This study focuses on MYB transcription factors(TFs)that regulate the biosynthesis of glucosinolates(GSL)in Brassicaceae,aiming to systematically analyze their evolutionary dynamics,chromosomal distribution,collinearity,and gene expression patterns to reveal the evolutionary mechanisms of the glucosinolate biosynthetic pathway and its role in plant adaptation.[Method]A species tree was constructed for 29 chromosome-level species to analyze the impact of whole-genome duplication events on the number of MYB TFs.MYB TFs related to glucosinolate biosynthesis were identified in 29 species,and a phylogenetic and domain structure analysis was performed on the relevant transcription factors in the tribe Brassiceae.Chromosomal localization analysis was conducted to study the distribution characteristics of these transcription factors on chromosomes.Collinearity analysis was performed to reveal the evolutionary conservation and differentiation of these genes among different species.Gene expression patterns were analyzed to study their tissue-specific expression.[Result]Whole-genome duplication events significantly increased the number of MYB TFs,especially in the tribe Brassiceae.A total of 322 MYB TFs related to glucosinolate biosynthesis were identified in 29 species,among which 176 were identified in Brassiceae tribe.The MYB TFs related to glucosinolate biosynthesis in Brassiceae tribe showed significant clustering on the phylogenetic tree,indicating that they retained similar structures and functions during evolution.Chromosomal localization analysis revealed that these transcription factors were mainly distributed at the ends of chromosomes,which may be related to the flexibility of their expression regulation.Collinearity analysis revealed the evolutionary conservation and differentiation of these genes among different species.Gene expression pattern analysis showed that they exhibited significant tissue-specific expression.[Conclusion]This study provides an important molecular basis for understanding the evolutionary mechanisms of the glucosinolate biosynthetic pathway in Brassicaceae and its role in plant adaptation,offers new perspectives for subsequent functional validation and application research.关键词
十字花科/芸薹族/硫苷/MYB转录因子/进化机制/合成途径/染色体/表达调控Key words
Brassicaceae/Brassiceae/glucosinolate/MYB transcription factors/evolutionary mechanism/biosynthetic pathway/chromosome/expression regulation分类
园艺学与植物营养学引用本文复制引用
李春金,何靖华,张欣瑶,曹睿,宋小明..十字花科植物调控硫苷合成MYB转录因子的生物信息学分析[J].东南园艺,2025,13(1):1-14,14.基金项目
国家自然科学基金项目(32172583) （32172583）
中央引导地方科技发展资金项目(246Z2509G) （246Z2509G）
河北省杰出青年基金项目(C2022209010) （C2022209010）
河北省省属高校基本科研业务费基金项目(JJC2024001) （JJC2024001）
