感染、炎症、修复2025，Vol.26Issue(2)：98-101,4.DOI:10.3969/j.issn.1672-8521.2025.02.005
依托咪酯对脓毒症大鼠急性肺损伤的保护作用及其机制的研究
Protective effect of etomidate on acute lung injury in septic rats and its mechanism
黄年丽 1王运星 1李英霞 1季兰馨 1金艺华1
作者信息
- 1. 佳木斯大学附属第一医院,黑龙江 佳木斯 154000
- 折叠
摘要
Abstract
Objective:To explore the protective effect of etomidate(ETO)on acute lung injury in septic rats and the relationship between its mechanism of action and the regulation of the nuclear factor-erythroid 2-related factor 2(Nrf2)/heme oxygenase 1(HO-1)signaling pathway.Methods:Forty clean male Sprague-Dawley rats were randomly divided into five groups:the blank control group,sham surgery group,sepsis group[cecal ligation and puncture(CLP)group,animal model of sepsis lung injury was prepared by CLP],CLP+ETO group,CLP+ETO+ML385 group(ML385 is Nrf2 inhibitor),with 8 rats in each group.The rats were acclimatized for one week.The blank control group received no special treatment,and the sham surgery group underwent sham surgery.The other groups were subjected to CLP to induce sepsis model and given corresponding drug intervention.Serum levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were measured by enzyme-linked immunosorbent assay(ELISA)18 days after the model was prepared.The pathological injury status of lung tissue was evaluated by hematoxylin-eosin staining,and the pathological injury score of lung tissue was performed.The protein expression levels of Nrf2 and HO-1 in the lung tissue were detected using Western Blot.Results:Etomidate alleviated lung edema and pathological changes in the lungs of rats induced by CLP,reduced the levels of serum inflammatory factors TNF-α and IL-1β(P＜0.05).Meanwhile,the expression of Nrf2 and HO-1 protein was upregulated(P＜0.05).In the CLP+ETO+ML385 group,the levels of TNF-α and IL-1β were increased,the pathological injury scores were increased,and Nrf2 and HO-1 protein expression levels were decreased(P＜0.05).Conclusions:Etomidate may exert its protective effect on sepsis-induced lung tissue damage by regulating the Nrf2/HO-1 signaling pathway.关键词
脓毒症/肺损伤,急性/依托咪酯/核转录因子红系2相关因子2/血红素加氧酶1Key words
Sepsis/Acute lung injury/Etomidate/Nuclear factor erythroid 2-related factor 2/Heme oxygenase 1分类
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黄年丽,王运星,李英霞,季兰馨,金艺华..依托咪酯对脓毒症大鼠急性肺损伤的保护作用及其机制的研究[J].感染、炎症、修复,2025,26(2):98-101,4.
