黑龙江八一农垦大学学报2025，Vol.37Issue(2)：23-31,9.DOI:10.3969/j.issn.1002-2090.2025.02.004
基于SYBR GreenⅡ的BVDV NS3基因实时荧光定量PCR检测方法的建立与应用
Establishment and Application of qRT-PCR Assay for BVDV NS3 Gene Based on SYBR GreenⅡ
摘要
Abstract
To establish a real-time fluorescent quantitative PCR(qRT-PCR)method for the detection of bovine viral diarrhea virus(BVDV)NS3 gene based on SYBR GreenⅡ,the qRT-PCR primers of BVDV NS3 gene were designed and screened,and the qRT-PCR detection method was established.Clinical bovine serum samples were also detected by CFX96 and pig early warning machine-2000.The results showed that one pair of primers with good specificity was selected.The minimum detectable values of CFX96 and pig early warning machine-2000 qRT-PCR standards were 1×101 copies·μL-1 and 1×102 copies·μL-1,respectively,with good specificity and repeatability,and the detection results of clinical serum samples were accurate and consistent.It provides technical means for BVDV detection.关键词
牛病毒性腹泻病毒/NS3基因/实时荧光定量PCR/SYBR GreenⅡKey words
bovine viral diarrhea virus/NS3 gene/qRT-PCR/SYBR GreenⅡ分类
畜牧业引用本文复制引用
李阳,张世勋,赫鸣睿,刘珊珊,岳山,刘宇,朱战波..基于SYBR GreenⅡ的BVDV NS3基因实时荧光定量PCR检测方法的建立与应用[J].黑龙江八一农垦大学学报,2025,37(2):23-31,9.基金项目
黑龙江省"揭榜挂帅"科技攻关项目,2023ZXJ02B03. （）
