黑龙江八一农垦大学学报2025，Vol.37Issue(2)：90-95,6.DOI:10.3969/j.issn.1002-2090.2025.02.013
基因工程菌pHY-P43-almA(mdhA)/B.subtilis WB800的构建及其石油烃降解效果研究
Construction of Genetic Engineered Bacteria pHY-P43-almA(mdhA)/B.subtilis WB800 and Its Degradation Efficiency of Petroleum Hydrocarbon
摘要
Abstract
In order to improve the efficiency of indigenous microorganisms in degrading petroleum hydrocarbons,the experiment was genetically modified by introducing exogenous genes almA and mdhA into Bacillus subtilis(B.subtilis)WB800 to construct a recom-binant body.At the same time,gas chromatography technology was used to determine the petroleum hydrocarbon content in the sewage sludge.The results showed that compared with the control group,the recombinant bacteria significantly improved the degradation rates of C12,C17 and shale oil sludge.The degradation rates of C12 and C17 by pHY-P43-almA(mdhA)/B.subtilis WB800 increased about 51.17%and 53.68%respectively,and for shale oil sludge increased by 18.26%.These results demonstrated that genetic engineering B.subtilis could effectively improve the degradation efficiency of petroleum hydrocarbon,especially for long-chain alkanes above C20,providing new methods and ideas for bioremediation of petroleum hydrocarbon pollution.关键词
基因工程菌/基因almA/mdhA/石油烃污染/生物修复Key words
genetically engineered bacteria/gene almA/mdhA/petroleum hydrocarbon pollution/bioremediation分类
资源环境引用本文复制引用
蔡昌福,张春龙,余丽芸,谢秀月,秦达,王嘉彬,曲艺,曹迪,侯喜林,毛立丰,沈江川..基因工程菌pHY-P43-almA(mdhA)/B.subtilis WB800的构建及其石油烃降解效果研究[J].黑龙江八一农垦大学学报,2025,37(2):90-95,6.基金项目
黑龙江省重点研发计划项目(GA21C028). （GA21C028）
