煤炭与化工2025，Vol.48Issue(4)：143-147,5.DOI:10.19286/j.cnki.cci.2025.04.030
HPLC法同时测定调胃丹中六种有效成分的含量
The content of six active ingredients in the stomach regulating pill determined by HPLC method at the same time
摘要
Abstract
In order to establish a high-performance liquid chromatography method for the simultaneous determination of the active ingredients of trifoliate orange,clove,incense tree and magnolia officinalis in the prescription of tiaoweidan by using COSMOSIL 5C18-MS-Ⅱ.(4.6 ID × 250 mm,5 μ m)Column.The mobile phase was methanol-water with gradient elution.The volume flow rate was 1.0 mL/min,the detection wavelength was 220 nm,the column temperature was 30 ℃,and the injection volume was 10 μL.Results showed that Neohesperidin,eugenol,lignolide,dehydrolignolide,honokiol and magnolol had good linear relationship and good precision,repeatability and stability in their linear range.The average recoveries were 99.27％～100.82％,and RSD were less than 2.0％.The method for the determination of six active components in tiaoweidan is rapid,simple,reproducible and specific,which can be used for the quality control of tiaoweidan.关键词
调胃丹/HPLC/含量测定/质量控制Key words
tiaoweidan/HPLC/content determination/quality control分类
化学化工引用本文复制引用
张鑫,杨晓霞,常黎明,柴国林..HPLC法同时测定调胃丹中六种有效成分的含量[J].煤炭与化工,2025,48(4):143-147,5.基金项目
甘肃省重点研发(编号2021GSMPA-KL01、2021GSMPA-KL02) （编号2021GSMPA-KL01、2021GSMPA-KL02）
