中医学报2025，Vol.40Issue(5)：1108-1115,8.DOI:10.16368/j.issn.1674-8999.2025.05.178
灯盏花素调节PI3K/AKT通路增强人非小细胞肺癌A549细胞顺铂化疗敏感性
Calendulin Enhancing Cisplatin Chemosensitivity in Human Non-small Cell Lung Cancer A549 Cells by Regulating PI3K/AKT pathway
摘要
Abstract
Objective:To observe the effect of lanugoin on the sensitivity of human non-small cell lung cancer A549 cells to cisplatin chemotherapy,and to explore the potential mechanism of action based on phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)pathway.Methods:A549 cells were intervened with 0,2,4,8,16,and 32 μmol·L-1 luciferin and 0,1,2,4,8,and 16 μmol·L-1 cispla-tin,and the cell proliferation viability was detected by MTT assay,and the semi-inhibitory concentrations IC50 luciferin and IC50 cis-platin were calculated.The A549 cells were divided into blank group,lamparicin group,cisplatin group,lamparicin+cisplatin group,and lamparicin+cisplatin+LY294002 group.After 48 h of culture,cell viability was detected by MTT assay,cell clone formation ability was detected by plate cloning assay,cell apoptosis was detected by flow cytometry,cell expression levels of PI3K and AKT genes were detec-ted by RT-PCR,and cell PI3K,AKT,B-cell lymphoma-2(Bcl-2),Bcl-2-related X protein(Bax),cysteine protease(Caspase)-9,Cleaved Caspase-9,Caspase-3,Cleaved Caspase-3 protein expression levels.Results:The inhibitory effects of both lamprophyllin and cisplatin on the proliferation viability of A549 cells were dose-dependent,with IC50 lamprophyllin and IC50 cisplatin being14.36 μmol·L-1 and5.72 μmol·L-1,respectively.compared with the blank group,the proliferation viability and the number of clone formation of the cells in the rest of the group were reduced,the apoptosis rate was elevated,and the levels of PI3K mRNA and AKT mRNA were decreased(P<0.05).Compared with the lanugin group or the cisplatin group,the cell proliferation vigor,the number of clone formation decreased,the apoptosis rate increased,and the levels of PI3K mRNA and AKT mRNA decreased in the lanugin+cis-platin group and the lanugin+cisplatin+LY294002 group(P<0.05).Compared with the lanugin+cisplatin group,cell proliferation vigor,number of clone formation decreased,apoptosis rate increased,and PI3K mRNA,AKT mRNA levels decreased in the lanugin+cisplatin+LY294002 group(P<0.05).Compared with the blank group,the cellular PI3K,AKT,and Bcl-2 protein expression levels were reduced in the rest of the group,and the protein expression levels of Bax,Cleaved Caspase-9,Cleaved Caspase-3 and Bax/Bcl-2,Cleaved Caspase-9/Caspase-9,Cleaved Caspase-3/Caspase-3 ratio were elevated(P<0.05).Compared with the lanu-gin group or the cisplatin group,the cellular PI3K,AKT,and Bcl-2 protein expression levels were decreased in the lanugin+cisplatin group and the lanugin+cisplatin+LY294002 group,and the protein expression levels of Bax,Cleaved Caspase-9,and Cleaved Caspase-3 protein expression levels and the ratio of Bax/Bcl-2,Cleaved Caspase-9/Caspase-9,and Cleaved Caspase-3/Caspase-3 ratio were elevated(P<0.05).Compared with the lanugin+cisplatin group,the cellular PI3K,AKT,and Bcl-2 protein expression levels were decreased in the lanugin+cisplatin+LY294002 group,and the protein expression levels of Bax,Cleaved Caspase-9,Cleaved Caspase-3,and the ratios of Bax/Bcl-2,Cleaved Caspase-9/Caspase-3/Caspase-3 were increased(P<0.05).Conclu-sion:Lampetin may enhance the chemosensitivity of A549 cells by inhibiting cell proliferation and promoting apoptosis through inhibiting the PI3K/AKT pathway.关键词
非小细胞肺癌/灯盏花素/顺铂/A549 细胞/PI3K/AKT通路/化疗Key words
non-small cell lung cancer/calendulin/cisplatin/A549 cell/PI3K/AKT pathway/chemotherapy分类
医药卫生引用本文复制引用
韦辉,王天珩,高世奇..灯盏花素调节PI3K/AKT通路增强人非小细胞肺癌A549细胞顺铂化疗敏感性[J].中医学报,2025,40(5):1108-1115,8.基金项目
河北省医学科学研究课题项目(20220506) （20220506）
